Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.

中文翻译：

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动态全场光学相干断层扫描：视网膜类器官的 3D 实时成像。

光学相干断层扫描提供了对活体组织的复杂结构进行成像的惊人机会，但缺乏功能信息。我们提出动态全场光学相干断层扫描作为一种对活体人类诱导多能干细胞衍生的视网膜类器官进行无创成像的技术。生成具有与细胞器运动相关的内源对比度的彩色图像，具有亚微米空间分辨率和毫秒时间分辨率，创造了一种通过其功能识别活组织中特定细胞类型的方法。