Nine yeast strains isolated from Latin American biodiversity were screened for ferulic acid (FA) consumption and conversion into aroma compounds such as vanillin, vanillic acid (VA), and 4‐vinylguaiacol (VG). Selected strains (Rhodotorula mucilaginosa UFMG‐CM‐Y3647, UFMG‐CM‐Y2190, UFMG‐CM‐Y665) were evaluated in flask experiments to investigate the influence of the pH media on bioconversion and a two‐step process was conducted to maximize the metabolites production. The effect of pH was found to be significantly important for FA bioconversion, as acidic conditions (pH < 6.0) improved VA accumulation, with highest production of 1.14 ± 0.02 and 1.25 ± 0.03 g/L shown by UFMG‐CM‐Y3647 and UFMG‐CM‐Y2190, respectively. The two‐step process favored 4‐VG production for most strains, being UFMG‐CM‐Y2190 the best producer, its cultures reaching 1.63 ± 0.09 g/L after 55 hr, showing a productivity of 29.59 ± 1.55 mg/(L·hr), as glucose affected the metabolites pool and redirected yeast metabolism. R mucilaginosa UFMG‐CM‐Y3647 was selected for scaled‐up cultivations in a 2‐L bioreactor, where pH‐controlled pH 5.5 and aeration of 2.5 vvm was found to be the best condition to improve VA productivity, attaining final concentrations of 1.20 ± 0.02 g/L⁻¹ (78% molar yield) and a productivity of 40.82 ± 0.57 mg/(L·hr).

中文翻译：

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拉丁美洲生物多样性新分离的酵母菌株将阿魏酸生物转化为芳香化合物

筛选从拉丁美洲生物多样性中分离的九种酵母菌株，以消耗阿魏酸 (FA) 并转化为芳香化合物，如香草醛、香草酸 (VA) 和 4-乙烯基愈创木酚 (VG)。选定的菌株（Rhodotorula mucilaginosaUFMG-CM-Y3647、UFMG-CM-Y2190、UFMG-CM-Y665）在烧瓶实验中进行了评估，以研究 pH 介质对生物转化的影响，并进行了两步过程以最大限度地提高代谢物的产量。发现 pH 值的影响对于 FA 生物转化非常重要，因为酸性条件（pH < 6.0）改善了 VA 的积累，UFMG-CM-Y3647 和 UFMG- CM-Y2190，分别。两步法有利于大多数菌株的 4-VG 生产，UFMG-CM-Y2190 是最好的生产者，其培养物在 55 小时后达到 1.63 ± 0.09 g/L，显示生产力为 29.59 ± 1.55 mg/(L·hr )，因为葡萄糖影响代谢物池并重定向酵母代谢。粘液红霉选择 UFMG-CM-Y3647 在 2 L 生物反应器中进行放大培养，其中 pH 控制的 pH 值 5.5 和 2.5 vvm 的曝气被发现是提高 VA 生产力的最佳条件，最终浓度为 1.20 ± 0.02 g /L ^-1 (78% 摩尔产率) 和 40.82 ± 0.57 mg/(L·hr) 的产率。