Tunable polymer microgel particles and their study using microscopy and real‐time deformability cytometry,Journal of Polymer Science

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Tunable polymer microgel particles and their study using microscopy and real‐time deformability cytometry
Journal of Polymer Science ( IF 3.9 ) Pub Date : 2020-08-17 , DOI: 10.1002/pol.20200274
Sheilan Sinjari ₁ , Jessica S. Freitag ₂ , Christoph Herold ₃ , Oliver Otto _{3,

4} , David M. Smith _{2,

5,

6} , Harald D. H. Stöver ₁

Affiliation

We report the preparation and mechanical properties of highly swellable, spherical polymer microgels synthesized by precipitation copolymerization of divinylbenzene‐55 (DVB), 4‐methylstyrene (4MS), and maleic anhydride (MA) at different cross‐linker contents, in a range of methylethylketone (MEK) and heptane solvent mixtures. Microgels were characterized by optical and confocal microscopy, and their mechanical properties tested using real‐time deformability cytometry (RT‐DC), a technique developed to analyze cell properties by measuring deformation under shear stress. Hydrolysis of anhydride groups gave microgels with diameters ranging from 10 to 22 μm when swollen in saline, depending on vol% MEK and cross‐linker loading. Young's moduli of the microgels could be tuned from 0.8 to 10 kPa by adjusting cross‐linker content and MEK/heptane solvent composition, showing an inverse relationship between the effects of vol% MEK and %DVB on microgel properties. These microgels also show strain‐stiffening in response to increasing shear stresses. Extension of the RT‐DC method to the study of polymer colloids thus enables high‐throughput analysis of microgels with tunable mechanical characteristics.

中文翻译：

可调聚合物微凝胶颗粒及其使用显微镜和实时可变形细胞术的研究

我们报告了在不同交联剂含量下，通过二乙烯苯-55（DVB），4-甲基苯乙烯（4MS）和马来酸酐（MA）的沉淀共聚反应合成的高度可膨胀的球形聚合物微凝胶的制备和力学性能。甲基乙基酮（MEK）和庚烷溶剂的混合物。通过光学和共聚焦显微镜对微凝胶进行了表征，并使用实时可变形细胞计数法（RT-DC）测试了微凝胶的力学性能，该技术是通过测量剪切应力下的变形来分析细胞特性的技术。当在盐水中溶胀时，酸酐基团的水解产生直径为10至22μm的微凝胶，这取决于MEK的体积百分比和交联剂的负载量。微凝胶的杨氏模量可以从0调整。通过调节交联剂含量和MEK /庚烷溶剂组成，可在8至10 kPa之间显示vol％MEK和％DVB对微凝胶性能的反比关系。这些微凝胶在剪切应力增加时也表现出应变刚度。因此，将RT-DC方法扩展到聚合物胶体的研究就可以对具有可调机械特性的微凝胶进行高通量分析。

更新日期：2020-08-17

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