The aims of this preliminary study were (a) to evaluate the effect of replacing total fishmeal (FM) protein (crude protein, 70.7% CP) with terrestrial animal by‐products (porcine meat and poultry by‐products; 59.7% CP and 61.6% respectively) and plant meal (soya bean concentrate and corn gluten; CP = 47.3% and 72.7% respectively) on the gut microbiota of Lutjanus guttatus juveniles and (b) to assess the effect of intermittent feeding in transient microbiota. Organisms (106 ± 12 g) were fed a FM, terrestrial animal by‐products (PPM) or plant (VEGM)‐based diet for ten days; afterwards, they were fed FM diet for ten more days. Samples were analysed using 16S rRNA taxonomic fingerprinting, and function profile analysis was determined by PICRUSt. Serratia and Burkholderia were representative of resident microbiota, while Photobacterium and Vibrio were the predominant genera in transient microbiota. Nor the total FM replacement by PPM and VEGM, nor the intermittent feeding, affected the predominant bacterial populations in the snapper gut microbiota, but significant changes in the amino acid metabolism pathway were observed in PPM group. This research might provide a guideline to define alternative ingredients to fishmeal in order to develop more sustainable diets without negatively affecting the intestinal microbiota composition.

中文翻译：

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初步研究不同饮食来源的鱼粉替代品对斑点玫瑰鲷鱼幼鱼肠道菌群的影响（Lutjanus guttatus Steindachner，1869年）

这项初步研究的目的是（a）评价用陆生动物副产品（猪肉和家禽副产品； 59.7％CP和61.6）代替总鱼粉（FM）蛋白（粗蛋白，CP为70.7％）的效果％）和植物粉（大豆浓缩物和玉米面筋; CP分别为47.3％和72.7％）对lututus guttatus幼鱼肠道菌群的影响，（b）评估间歇性喂食对瞬时菌群的影响。给动物（106±12克）饲喂FM，陆生动物副产品（PPM）或植物（VEGM）为基础的饮食，持续10天；之后，又给他们喂了FM饮食十天。使用16S rRNA分类指纹对样品进行分析，并通过PICRUSt确定功能谱分析。沙雷氏菌和伯克霍尔德氏菌细菌是常驻菌群的代表，而细菌和弧菌是瞬时菌群的主要属。PPM和VEGM不能完全替代FM，也不能间歇喂食，不会影响鲷鱼肠道菌群中的主要细菌种群，但是PPM组的氨基酸代谢途径发生了显着变化。这项研究可能会为定义鱼粉的替代成分提供指导，以开发更可持续的饮食，而不会负面影响肠道菌群的组成。