Slr4, a newly identified S-layer protein from marine Gammaproteobacteria, is a major biofilm matrix component.,Molecular Microbiology

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Slr4, a newly identified S-layer protein from marine Gammaproteobacteria, is a major biofilm matrix component.
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-08-17 , DOI: 10.1111/mmi.14588
Sura Ali ₁ , Benjamin Jenkins ₁ , Jiujun Cheng _{1,

2} , Briallen Lobb ₁ , Xin Wei ₁ , Suhelen Egan ₃ , Trevor C Charles _{1,

2} , Brendan J McConkey ₁ , John Austin ₄ , Andrew C Doxey ₁

Affiliation

S‐layers are paracrystalline proteinaceous lattices that surround prokaryotic cells, forming a critical interface between the cells and their extracellular environment. Here, we report the discovery of a novel S‐layer protein present in the Gram‐negative marine organism, Pseudoalteromonas tunicata D2. An uncharacterized protein (EAR28894) was identified as the most abundant protein in planktonic cultures and biofilms. Bioinformatic methods predicted a beta‐helical structure for EAR28894 similar to the Caulobacter S‐layer protein, RsaA, despite sharing less than 20% sequence identity. Transmission electron microscopy revealed that purified EAR28894 protein assembled into paracrystalline sheets with a unique square lattice symmetry and a unit cell spacing of ~9.1 nm. An S‐layer was found surrounding the outer membrane in wild‐type cells and completely removed from cells in an EAR28894 deletion mutant. S‐layer material also appeared to be “shed” from wild‐type cells and was highly abundant in the extracellular matrix where it is associated with outer membrane vesicles and other matrix components. EAR28894 and its homologs form a new family of S‐layer proteins that are widely distributed in Gammaproteobacteria including species of Pseudoalteromonas and Vibrio, and found exclusively in marine metagenomes. We propose the name Slr4 for this novel protein family.

中文翻译：

Slr4 是一种新发现的来自海洋 Gammaproteobacteria 的 S 层蛋白，是主要的生物膜基质成分。

S 层是围绕原核细胞的准结晶蛋白质晶格，在细胞与其细胞外环境之间形成关键界面。在这里，我们报告了存在于革兰氏阴性海洋生物Pseudoalteromonas tunicata D2中的新型 S 层蛋白的发现。一种未表征的蛋白质 (EAR28894) 被确定为浮游培养物和生物膜中最丰富的蛋白质。生物信息学方法预测EAR28894类似于β-螺旋结构柄杆菌S 层蛋白 RsaA，尽管共享不到 20% 的序列同一性。透射电子显微镜显示，纯化的 EAR28894 蛋白组装成具有独特方形晶格对称性和~9.1 nm 晶胞间距的次晶片。在野生型细胞的外膜周围发现了一个 S 层，并在 EAR28894 缺失突变体中从细胞中完全去除。S 层材料也似乎是从野生型细胞“脱落”的，并且在细胞外基质中高度丰富，在那里它与外膜囊泡和其他基质成分相关。EAR28894 及其同源物形成了一个新的 S 层蛋白家族，广泛分布在 Gammaproteobacteria 中，包括Pseudoalteromonas和Vibrio，并且仅在海洋宏基因组中发现。我们为这个新的蛋白质家族提议命名为 Slr4。

更新日期：2020-08-17

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