Swellable Gelatin Methacryloyl Microneedles for Extraction of Interstitial Skin Fluid toward Minimally Invasive Monitoring of Urea,Macromolecular Bioscience

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Swellable Gelatin Methacryloyl Microneedles for Extraction of Interstitial Skin Fluid toward Minimally Invasive Monitoring of Urea
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-08-17 , DOI: 10.1002/mabi.202000195
Daniela F S Fonseca ₁ , Paulo Cardoso Costa ₂ , Isabel F Almeida ₂ , Patrícia Dias-Pereira ₃ , Inês Correia-Sá ₄ , Verónica Bastos ₅ , Helena Oliveira ₅ , Carla Vilela ₁ , Armando J D Silvestre ₁ , Carmen S R Freire ₁

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Urea, the main nitrogenous waste product of protein metabolism, is eliminated almost exclusively by the kidney, and hence, displays considerable clinical significance in the assessment of kidney disorders. The aim of this study is to prepare and investigate the potential of swellable cross‐linked gelatin methacryloyl (c‐GelMA) microneedles (MNs) as a platform for minimally invasive extraction of interstitial skin fluid (ISF) toward straightforward point‐of‐care healthcare monitoring of renal complaints, by quantification of urea. c‐GelMA MNs are successfully prepared by photo‐cross‐linking and micromolding, faithfully replicating the master molds (387 ± 16 µm height, 200 µm base and 500 µm tip‐to‐tip distance). These MN patches display good mechanical properties, withstanding more than 0.15 N per needle without breaking. Ex vivo skin insertion assays reveal that the MNs penetrate up to 237 µm depth, reaching the dermis, where they should extract ISF considering a real application. In an in vitro application using an agarose skin model system, the c‐GelMA MNs are able to efficiently recover urea (>98%). Additionally, these MNs exhibit noncytotoxic effects toward human keratinocytes. These findings suggest that c‐GelMA MNs are promising devices for sampling ISF and offline analysis of urea, opening new avenues for simple point‐of‐care healthcare monitoring.

中文翻译：

可膨胀明胶甲基丙烯酰微针用于提取间质皮肤液以微创监测尿素

尿素是蛋白质代谢的主要含氮废物，几乎完全由肾脏清除，因此在评估肾脏疾病方面具有相当大的临床意义。本研究的目的是准备和研究可膨胀的交联明胶甲基丙烯酰 (c-GelMA) 微针 (MNs) 作为微创提取间质皮肤液 (ISF) 的平台的潜力，以实现直接的即时医疗保健通过定量尿素来监测肾脏疾病。c-GelMA MNs 通过光交联和微成型成功制备，忠实地复制了母模（387 ± 16 µm 高度，200 µm 底部和 500 µm 尖端到尖端距离）。这些 MN 贴片显示出良好的机械性能，每针可承受超过 0.15 N 的压力而不会断裂。离体皮肤插入试验表明，MNs 可穿透 237 µm 深度，到达真皮层，考虑到实际应用，它们应该在那里提取 ISF。在使用琼脂糖皮肤模型系统的体外应用中，c-GelMA MNs 能够有效地回收尿素 (>98%)。此外，这些 MNs 对人角质形成细胞表现出非细胞毒性作用。这些发现表明，c-GelMA MNs 是用于采样 ISF 和离线分析尿素的有前途的设备，为简单的即时医疗保健监测开辟了新途径。这些 MNs 对人角质形成细胞表现出非细胞毒性作用。这些发现表明，c-GelMA MNs 是用于采样 ISF 和离线分析尿素的有前途的设备，为简单的即时医疗保健监测开辟了新途径。这些 MNs 对人角质形成细胞表现出非细胞毒性作用。这些发现表明，c-GelMA MNs 是用于采样 ISF 和离线分析尿素的有前途的设备，为简单的即时医疗保健监测开辟了新途径。

更新日期：2020-10-12

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