Comparison of the biochemical properties and thermal inactivation of polyphenol oxidase from three lily bulb cultivars,Journal of Food Biochemistry

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Comparison of the biochemical properties and thermal inactivation of polyphenol oxidase from three lily bulb cultivars
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-08-17 , DOI: 10.1111/jfbc.13431
Xinyu Wang _{1,

2} , Lvzhu Yang _{1,

2} , Jie Liu _{1,

2} , Rongrong Wang ₃ , Qun Zhang _{1,

2,

4} , Yang Shan _{1,

2,

4} , Shenghua Ding _{1,

2,

4}

Affiliation

The biochemical properties and thermal inactivation of polyphenol oxidase (PPO) from three main planted lily cultivars in China, namely, Lilium lancifolium Thunb, Lilium brownie var. viridulum, and Lilium davidii var. unicolor cotton were evaluated. Data indicate that the PPO from three cultivars showed two optimum pH levels of 4.0 and 6.5–7.0 and temperature of 15°C and exhibited the highest affinity toward 4‐methylcatechol. However, this enzyme did not exhibit monophenolase activity. Thiourea and L‐cysteine were more effective than other inhibitors. The enzymatic activity of L. lancifolium Thunb PPO crude extract was higher than that of L. brownie var. viridulum and L. davidii var. unicolor cotton. For thermal inactivation, L. davidii var. unicolor cotton PPO showed the best thermal resistance at 65–75°C, and L. lancifolium Thunb showed stability at 45°C. The deactivation of the three types of PPO followed the first‐order reaction kinetics, and the activation energy (E_a) was 144.28, 138.00, and 107.12 kJ/mol for L. lancifolium Thunb PPO, L. brownie var. viridulum PPO, and L. davidii var. unicolor cotton PPO, respectively.

中文翻译：

三个百合品种多酚氧化酶的生化特性及热灭活比较

我国3个主栽百合品种Lilium lancifolium Thunb、 Lilium Brownie var.的生化特性及多酚氧化酶(PPO)热失活研究viridulum和Lilium davidii var.评估单色棉。数据表明，三个品种的 PPO 显示出 4.0 和 6.5-7.0 两个最佳 pH 水平以及 15°C 的温度，并且对 4-甲基儿茶酚表现出最高的亲和力。然而，该酶不表现出单酚酶活性。硫脲和L-半胱氨酸比其他抑制剂更有效。 L. lancifolium Thunb PPO 粗提物的酶活性高于L. Brownie var.。 viridulum和L. davidii var.单色棉。对于热灭活， L. davidii var.单色棉PPO在65-75°C表现出最好的耐热性， L. lancifolium Thunb在45°C表现出稳定性。三种类型PPO的失活均遵循一级反应动力学， L. lancifolium Thunb PPO、 L. Brownie var. 的活化能（ E _a ）分别为144.28、138.00和107.12 kJ/mol。 viridulum PPO 和L. davidii var.分别为单色棉PPO。

更新日期：2020-10-11

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