Members of the somatotrophic axis, especially GH and IGF1, are essential for growth. The association between GH polymorphisms and growth traits was numerously studied in cattle; however, no data are available for such association studies in buffalo. Therefore, this study was conducted to screen for polymorphisms in the GH gene and to study their putative association with growth traits in 200 Egyptian buffaloes. Polymerase chain reaction single-strand conformation polymorphism and sequencing were applied to look for polymorphisms in 3 loci spanning all exons and introns of buffalo GH. The C (MspI+) >T (MspI-) SNP in intron3, which is well known in cattle, was not detected in the examined buffaloes. However, 2 missense mutations were detected in exon5: one previously detected p.Leu153Val SNP, with very low frequencies for the mutant (Val) allele and one novel p.Asn174His SNP. At weaning age, the p.Leu153Val SNP was significantly associated with weaning body weight and gain with the positive effect of the wild allele (Leu) and higher GH serum, mRNA, and protein levels in animals with Leu/Asn and Leu/His haplotypes. At yearling age, the 2 SNPs associated with yearling weight and gain with positive effect for the mutant (Val and His) alleles with increased GH, and IGF1 serum, mRNA, and protein and GHR mRNA and protein levels in animals with Val/Asn haplotype. Therefore, the selection of Egyptian buffaloes with the Val/Asn haplotype could improve the growth traits of Egyptian buffaloes at yearling age which is the target age for perfect growing.

生长激素轴的成员，尤其是 GH 和 IGF1，对生长至关重要。GH多态性与生长性状之间的关联在牛身上进行了大量研究。然而，没有数据可用于布法罗的此类关联研究。因此，本研究旨在筛选GH基因的多态性，并研究它们与 200 头埃及水牛的生长性状的推定关联。应用聚合酶链反应单链构象多态性和测序来寻找跨越水牛GH所有外显子和内含子的3个基因座的多态性. 在牛中众所周知的内含子3 中的C (MspI+) > T (MspI-) SNP 在检查的水牛中未检测到。然而，在外显子 5 中检测到 2 个错义突变：一个先前检测到的 p.Leu153Val SNP，突变 (Val) 等位基因的频率非常低，一个新的 p.Asn174His SNP。在断奶年龄，p.Leu153Val SNP 与断奶体重和增加显着相关，野生等位基因 (Leu) 的积极作用和 Leu/Asn 和 Leu/His 单倍型动物的 GH 血清、mRNA 和蛋白质水平较高. 在周龄，与周岁体重和增重相关的 2 个 SNP 对具有 Val/Asn 单倍型动物的 GH 和 IGF1 血清、mRNA 和蛋白质以及 GHR mRNA 和蛋白质水平增加的突变（Val 和 His）等位基因具有积极影响. 所以，