In the present work, we investigated the interaction of flavonoids (quercetin, naringenin and catechin) with cellular and artificial membranes. The flavonoids considerably inhibited membrane lipid peroxidation in rat erythrocytes treated with tert-butyl hydroperoxide (700 μM), and the IC₅₀ values for prevention of this process were equal to 9.7 ± 0.8 μM, 8.8 ± 0.7 μM, and 37.8 ± 4.4 μM in the case of quercetin, catechin and naringenin, respectively, and slightly decreased glutathione oxidation. In isolated rat liver mitochondria, quercetin, catechin and naringenin (10–50 μM) dose-dependently increased the sensitivity to Ca²⁺ ions - induced mitochondrial permeability transition. Using the probes TMA-DPH and DPH we showed that quercetin rather than catechin and naringenin strongly decreased the microfluidity of the 1,2-dimyristoyl-sn-glycero-3-phosphocholine liposomal membrane bilayer at different depths. On the contrary, using the probe Laurdan we observed that naringenin transfer the bilayer to a more ordered state, whereas quercetin dose-dependently decreased the order of lipid molecule packing and increased hydration in the region of polar head groups. The incorporation of the flavonoids, quercetin and naringenin and not catechin, into the liposomes induced an increase in the zeta potential of the membrane and enlarged the area of the bilayer as well as lowered the temperature and the enthalpy of the membrane phase transition. The effects of the flavonoids were connected with modification of membrane fluidity, packing, stability, electrokinetic properties, size and permeability, prevention of oxidative stress, which depended on the nature of the flavonoid molecule and the nature of the membrane.

在目前的工作中，我们研究了类黄酮（槲皮素，柚皮素和儿茶素）与细胞膜和人工膜的相互作用。类黄酮显着抑制了用叔丁基氢过氧化物（700μM）处理的大鼠红细胞中的膜脂质过氧化，并且用于预防该过程的IC ₅₀值分别为9.7±0.8μM，8.8±0.7μM和37.8±4.4μM。槲皮素，儿茶素和柚皮素的情况下，谷胱甘肽氧化略有降低。在离体大鼠肝线粒体中，槲皮素，儿茶素和柚皮素（10–50μM）剂量依赖性地增加了对Ca ²⁺的敏感性离子-诱导线粒体通透性转变。使用探针TMA-DPH和DPH，我们显示了槲皮素而不是儿茶素和柚皮素能在不同深度强烈降低1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱脂质体双层膜的微流动性。相反，使用探针Laurdan可以观察到柚皮苷将双层分子转移至更有序的状态，而槲皮素剂量依赖性地降低了极性分子团中脂质分子的堆积顺序并增加了水合作用。将类黄酮，槲皮素和柚皮素而不是儿茶素掺入脂质体中会引起膜的Zeta电位增加，并扩大双层面积，并降低温度和膜相转变的焓。