Antioxidant compounds were produced from submerged fermentation using Botryosphaeria dothidea. Sonication times (1–15 min) and agitations (0–150 rpm) applied to different fermentation phases (2–7 days) were evaluated for their effect on the broth antioxidant activity. In approximately 70% of assays performed with ultrasound, the antioxidant activity of the broth containing exocellular metabolites was higher than the control (without sonication), reaching a maximum value of 96%. The solid fraction (biomass after supernatant removal) corresponding to the best assay of antioxidant activity was submitted to extractions with water, ethanol or ethyl acetate to recover intracellular metabolites. The yields of extracts were 3.7 ± 2.6 wt %, 20.6 ± 3.9 wt % and 32.0 ± 4.2 wt % for ethyl acetate, ethanol and water, respectively. The use of ethanol could provide a larger number of compounds such as pentadecanoic acid, ergosta-5,8,22-trien-3-ol, and 1,4-diaza-2,5-dioxobicyclo [4.3.0] nonane. Both the supernatant and solid fractions revealed some bioactive compounds with promising antioxidant activity with the potential to replace some synthetic antioxidants.

中文翻译：

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超声波对深水发酵生产 Botryosphaeria dothidea 抗氧化代谢物的影响

使用 Botryosphaeria dothidea 通过深层发酵生产抗氧化化合物。评估应用于不同发酵阶段（2-7 天）的超声处理时间（1-15 分钟）和搅拌（0-150 rpm）对肉汤抗氧化活性的影响。在大约 70% 的超声检测中，含有胞外代谢物的肉汤的抗氧化活性高于对照（无超声处理），最大值为 96%。与抗氧化活性的最佳测定相对应的固体部分（去除上清液后的生物质）用水、乙醇或乙酸乙酯进行提取以回收细胞内代谢物。乙酸乙酯、乙醇和水的提取物产率分别为 3.7 ± 2.6 wt%、20.6 ± 3.9 wt% 和 32.0 ± 4.2 wt%。使用乙醇可以提供更多的化合物，例如十五烷酸、ergosta-5,8,22-trien-3-ol 和 1,4-diaza-2,5-dioxobicyclo [4.3.0] 壬烷。上清液和固体部分都揭示了一些具有良好抗氧化活性的生物活性化合物，有可能取代一些合成抗氧化剂。