H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was associated with gosling growth retardation syndrome growth retardation syndrome complicated by visceral urate deposition. However, there is no accurate and high throughput real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) available for the rapid and highly sensitive identification of H146-like GCV. In this study, a pair of specific primers and a TaqMan minor groove binder (MGB) probe were designed based on a conserved region in the nonstructural (NS) gene sequence. The TaqMan-MGB probe-based one-step qRT-PCR assay was capable of detecting quite low number of targeting nucleic acid as low as 5.07 copies/μL and had excellent intra-assay and inter-assay repeatability with the coefficient of variation (CV) value from 0.558% to 1.293%. The assay was highly specific for H146-like GCV, without cross-reactions with other non-targeted goose-origin viruses, and 62 suspicious tissue samples infected with H146-like GCV from different regions of Fujian Province were used in this study to verify the feasibility and effectiveness of this assay in clinical diagnosis. The results indicated that our assay for the diagnosis and quantification of H146-like GCV was highly sensitive and specific, and should provide a reliable real-time tool for epidemiological and pathogenetic study of H146-like GCV infection, enabling researchers to better understand the epidemiology and clinical presentation of this disease.

H146状鹅源杯状病毒（H146状GCV）是一种新型的杯状病毒科中的家庭成员Sanovirus幼鹅生长迟缓综合征伴有内脏尿酸盐沉积的生长迟缓综合征。但是，没有准确，高通量的实时定量逆转录聚合酶链反应（qRT-PCR）可用于快速和高度灵敏的H146样GCV鉴定。在这项研究中，基于非结构（NS）基因序列中的保守区，设计了一对特异性引物和TaqMan小沟结合剂（MGB）探针。基于TaqMan-MGB探针的一步式qRT-PCR分析能够检测极低数量的靶向核酸，低至5.07拷贝/μL，并且具有出色的批内和批间重复性，变异系数（CV ）值从0.558％降至1.293％。该测定对类似H146的GCV具有高度特异性，该研究未与其他非靶向鹅源病毒发生交叉反应，并使用了来自福建省不同地区的62株感染H146样GCV的可疑组织样本，以验证该测定法在临床诊断中的可行性和有效性。结果表明，我们对H146样GCV的诊断和定量测定具有很高的灵敏度和特异性，应为H146样GCV感染的流行病学和致病性研究提供可靠的实时工具，使研究人员能够更好地了解流行病学和这种疾病的临床表现。本研究使用了来自福建省不同地区的H146样GCV感染的62个可疑组织样本，验证了该方法在临床诊断中的可行性和有效性。结果表明，我们对H146样GCV的诊断和定量测定具有很高的灵敏度和特异性，应为H146样GCV感染的流行病学和致病性研究提供可靠的实时工具，使研究人员能够更好地了解流行病学和这种疾病的临床表现。本研究使用了来自福建省不同地区的H146样GCV感染的62个可疑组织样本，验证了该方法在临床诊断中的可行性和有效性。结果表明，我们对H146样GCV的诊断和定量测定具有很高的灵敏度和特异性，应为H146样GCV感染的流行病学和致病性研究提供可靠的实时工具，使研究人员能够更好地了解流行病学和这种疾病的临床表现。