Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-08-16 , DOI: 10.1016/j.diagmicrobio.2020.115168 Juan V Mulet Bayona 1 , Carme Salvador García 1 , Nuria Tormo Palop 1 , Concepción Gimeno Cardona 2
A shift to Candida non-albicans infections has been noted during the last years, and the emergence of multi-resistant Candida auris has complicated their management. The aim of this study was first to compare the performance of the novel chromogenic medium CHROMagar™ Candida Plus (CHROMagar, France) with CHROMagar™ Candida (Becton Dickinson, Germany) for the presumptive identification of Candida species; and then, to evaluate its utility in the detection of C. auris in surveillance samples. CHROMagar™ Candida Plus showed a good performance compared with the reference medium CHROMagar™ Candida. Sensitivity and specificity were 100% in both media for tested species at 48 h of incubation, except for Candida glabrata and Candida lusitaniae. Furthermore, the new medium allows a reliable presumptive identification of C. auris, as a new specific color for this species is assigned (light blue with a blue halo), obtaining a sensitivity and specificity of 100% at 36 h of incubation.
中文翻译:
新型念珠菌发色培养基的评价。鉴定并与CHROMagar™念珠菌进行比较,以检测监控样品中的金葡菌。
在换档念珠菌非白色念珠菌感染已在过去几年中已经注意到,和多重耐药的出现念珠菌耳具有复杂的管理。这项研究的目的是首先比较新型显色培养基CHROMagar™Candida Plus(法国CHROMagar)和CHROMagar™Candida(德国Becton Dickinson)在推定念珠菌物种方面的性能。然后评估其在监测样本中检测金黄色葡萄球菌的效用。与参考培养基CHROMagar™Candida相比,CHROMagar™Candida Plus表现出良好的性能。在培养48小时后,两种培养基对被测物种的敏感性和特异性均为100%,除了光滑念珠菌和绿假丝酵母。此外,新的培养基可以可靠地推测金黄色葡萄球菌,因为为该物种指定了新的特定颜色(浅蓝色和蓝色光晕),在孵育36小时后可获得100%的灵敏度和特异性。