Bombyx mori cytoplasmic polyhedrosis virus (BmCPV)that belongs to the genus Cypovirus in the family of Reoviridae is one of the problematic pathogens in sericulture. In our previous study, we have found that lipid-related constituents in the host cellular membrane are associated with the BmCPV life cycle. It is important to note that the lipids not only affect the cellular biological processes, they also impact the virus life cycle. However, the intracellular lipid homeostasis in BmN cells after BmCPV infection remains unclear. Here, the lipid metabolism in BmCPV-infected BmN cells was studied by lipidomics analysis. Our results revealed that the intracellular lipid homeostasis was disturbed in BmN cells upon BmCPV infection. Major lipids constituents in cellular membrane were found to be significantly induced upon BmCPV infection, which included triglycerides, phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, phospholipids, glucoside ceramide, monoetherphosphatidylcholin, ceramide, ceramide phosphoethanolamine and cardiolipin. Further analysis of the pathways related to these altered lipids (such as PE and PC) showed that glycerophospholipid metabolism was one of the most enriched pathways. These results suggested that BmCPV may manipulate the lipid metabolism of cells for their own interest. The findings may facilitate a better understanding of the roles of lipid metabolic changes during virus infection in future studies.

家蚕胞质多角体病毒(BmCPV) 属于呼肠孤病毒科Cypovirus 属，是养蚕中的问题病原体之一。在我们之前的研究中，我们发现宿主细胞膜中的脂质相关成分与 BmCPV 生命周期有关。值得注意的是，脂质不仅会影响细胞生物学过程，还会影响病毒的生命周期。然而，BmCPV 感染后 BmN 细胞的细胞内脂质稳态仍不清楚。在这里，通过脂质组学分析研究了 BmCPV 感染的 BmN 细胞中的脂质代谢。我们的研究结果表明，在 BmCPV 感染后，BmN 细胞中的细胞内脂质稳态受到干扰。发现细胞膜中的主要脂质成分在 BmCPV 感染后被显着诱导，其中包括甘油三酯、磷脂酰胆碱、磷脂酰乙醇胺、鞘磷脂、磷脂、葡萄糖苷神经酰胺、单醚磷脂酰胆碱、神经酰胺、神经酰胺磷酸乙醇胺和心磷脂。对与这些改变的脂质（如 PE 和 PC）相关的途径的进一步分析表明，甘油磷脂代谢是最丰富的途径之一。这些结果表明，BmCPV 可能会为了自己的利益而操纵细胞的脂质代谢。这些发现可能有助于在未来的研究中更好地理解病毒感染期间脂质代谢变化的作用。对与这些改变的脂质（如 PE 和 PC）相关的途径的进一步分析表明，甘油磷脂代谢是最丰富的途径之一。这些结果表明，BmCPV 可能会为了自己的利益而操纵细胞的脂质代谢。这些发现可能有助于在未来的研究中更好地理解病毒感染期间脂质代谢变化的作用。对与这些改变的脂质（如 PE 和 PC）相关的途径的进一步分析表明，甘油磷脂代谢是最丰富的途径之一。这些结果表明，BmCPV 可能会为了自己的利益而操纵细胞的脂质代谢。这些发现可能有助于在未来的研究中更好地理解病毒感染期间脂质代谢变化的作用。