Plant resistance and leaf chemical characteristic jointly shape phyllosphere bacterial community,World Journal of Microbiology and Biotechnology

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Plant resistance and leaf chemical characteristic jointly shape phyllosphere bacterial community
World Journal of Microbiology and Biotechnology ( IF 4.0 ) Pub Date : 2020-08-16 , DOI: 10.1007/s11274-020-02908-0
Tian Xueliang ₁ , Xu Dan ₂ , Sun Tingting ₁ , Zhao Songyu ₁ , Li Ying ₃ , Wang Diandong ₂

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Phyllosphere bacteria have an important role in plant growth and resistance to pathogen infection and are partially influenced by plant genotype and leaf environment. How plant resistance to pathogens and leaf chemical characteristics shape the phyllosphere bacterial communities is unclear. In this study, the phyllosphere bacterial communities of maize hybrids with various resistance to Setosphaeria turcica were compared using the high-throughput sequencing and large-scale culturing methods. The results showed that Shannon and Simpson indices of phyllosphere bacterial communities were markedly higher in the highly resistant hybrid (HR) compared with the susceptible one. Hierarchical clustering analysis, unweighted UniFrac principal component analysis (PCoA) and the analysis of similarities (ANOSIM) demonstrated that the phyllosphere bacterial communities were significantly distinct between resistant and susceptible hybrids. The redundancy analysis (RDA) demonstrated that leaf chemical characteristics, including nitrogen and phosphorus concentration, and disease resistance play an important role in shaping the phyllosphere bacterial community. Linear discriminant effect size (LEfSe) analysis indicated that Bacillus, Pseudomonas and Tumebacillus were the biomarker species in the phyllosphere of HR. Biocontrol bacteria against S. turcica (such as Pseudomonas and Bacillus) were isolated from the phyllosphere of HR by large-scale culturing. The work contributes to understanding of the phyllosphere bacterial community assembly and provides a new clue to screening for strong biocontrol bacteria from HR and to facilitating future breeding efforts for enhancing disease resistance.

中文翻译：

植物抗性和叶片化学特征共同塑造叶际细菌群落

叶际细菌在植物生长和抗病原侵染中具有重要作用，部分受植物基因型和叶片环境的影响。植物对病原体的抗性和叶片化学特征如何塑造叶际细菌群落尚不清楚。在这项研究中，使用高通量测序和大规模培养方法比较了对 Setosphaeria turcica 具有各种抗性的玉米杂种的叶际细菌群落。结果表明，高抗杂种（HR）中叶际细菌群落的香农和辛普森指数明显高于易感杂种。层次聚类分析，未加权 UniFrac 主成分分析 (PCoA) 和相似性分析 (ANOSIM) 表明，叶际细菌群落在抗性和易感杂交种之间存在显着差异。冗余分析 (RDA) 表明，叶片化学特征（包括氮和磷浓度）和抗病性在塑造叶际细菌群落中起着重要作用。线性判别效应大小 (LEfSe) 分析表明，芽孢杆菌、假单胞菌和根瘤菌是 HR 叶际中的生物标志物种类。通过大规模培养从HR的叶际中分离出针对S. turcica的生防细菌（如假单胞菌和芽孢杆菌）。

更新日期：2020-08-16

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