The present study provides an efficient cryopreservation protocol for Tulipa tarda cultured in vitro. Apices were excised from bulblets cultivated on MS medium, supplemented with 60 or 90 g l^− 1 sucrose. Half of the bulblets were subjected to a cold treatment at 5 °C for 10 weeks, before exposure of the apices to loading solution (LS) and plant vitrification solution 2 (PVS2). Ten weeks after rewarming and culture on recovery medium, 100% regrowth rates were obtained for cold treated explants cultured on 60 g l^− 1 sucrose after 30 and 60 min exposure to PVS2. Cold treatment significantly improved the recovery rates of most of the cryopreserved apical meristems while an enrichment of the culture medium with higher sucrose concentration (90 g l^− 1) did not improve regrowth of the apices.

本研究为体外培养的郁金香提供了一种有效的低温保存方案。从在补充有60或90 gl ^-1蔗糖的MS培养基上培养的鳞茎上切下顶端。在将顶点暴露于加载溶液（LS）和植物玻璃化溶液2（PVS2）之前，将一半的小球在5°C下进行10周的冷处理。重新加热并在恢复培养基上培养后十周，在暴露于PVS2 30和60分钟后，在60gl ^-1蔗糖上培养的冷处理外植体获得100％的再生率。冷冻处理显着提高了大多数冷冻保存的根尖分生组织的回收率，同时富含蔗糖浓度更高的培养基（90 gl ^{− 1）}）并没有改善顶点的再生。