Applications of CRISPR in a Microbial Cell Factory: From Genome Reconstruction to Metabolic Network Reprogramming.,ACS Synthetic Biology

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Applications of CRISPR in a Microbial Cell Factory: From Genome Reconstruction to Metabolic Network Reprogramming.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-08-14 , DOI: 10.1021/acssynbio.0c00349
Yaokang Wu _{1,

2} , Yanfeng Liu _{1,

2} , Xueqin Lv _{1,

2} , Jianghua Li _{1,

2} , Guocheng Du ₂ , Long Liu _{1,

2}

Affiliation

The well-designed microbial cell factory finds wide applications in chemical, pharmaceutical, and food industries due to its sustainable and environmentally friendly features. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (CRISPR-Cas) systems have been developed into powerful tools to perform genome editing and transcriptional regulation in prokaryotic and eukaryotic cells. Accordingly, these tools are useful to build microbial cell factories not only by reconstructing the genome but also by reprogramming the metabolic network. In this review, we summarize the recent significant headway and potential uses of the CRISPR technology in the construction of efficient microbial cell factories. Moreover, the future perspectives on the improvement and upgradation of CRISPR-based tools are also discussed.

中文翻译：

CRISPR 在微生物细胞工厂中的应用：从基因组重建到代谢网络重编程。

精心设计的微生物细胞工厂因其可持续和环保的特点在化学、制药和食品行业得到广泛应用。最近，成簇的规则间隔短回文重复序列 (CRISPR) 和 CRISPR 相关蛋白 (CRISPR-Cas) 系统已发展成为在原核和真核细胞中进行基因组编辑和转录调控的强大工具。因此，这些工具不仅可以通过重建基因组，还可以通过重新编程代谢网络来构建微生物细胞工厂。在这篇综述中，我们总结了 CRISPR 技术在构建高效微生物细胞工厂方面的近期重大进展和潜在用途。而且，

更新日期：2020-09-20

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