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Comparative secretome of white‐rot fungi reveals co‐regulated carbohydrate‐active enzymes associated with selective ligninolysis of ramie stalks
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-08-14 , DOI: 10.1111/1751-7915.13647 Chunliang Xie 1 , Wenbing Gong 1 , Zuohua Zhu 1 , Yingjun Zhou 1 , Chao Xu 1 , Li Yan 1 , Zhenxiu Hu 1 , Lianzhong Ai 1, 2 , Yuande Peng 1
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2020-08-14 , DOI: 10.1111/1751-7915.13647 Chunliang Xie 1 , Wenbing Gong 1 , Zuohua Zhu 1 , Yingjun Zhou 1 , Chao Xu 1 , Li Yan 1 , Zhenxiu Hu 1 , Lianzhong Ai 1, 2 , Yuande Peng 1
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In the present research, Phanerochaete chrysosporium and Irpex Lacteus simultaneously degraded lignin and cellulose in ramie stalks, whereas Pleurotus ostreatus and Pleurotus eryngii could depolymerize lignin but little cellulose. Comparative proteomic analysis of these four white‐rot fungi was used to investigate the molecular mechanism of this selective ligninolysis. 292 proteins, including CAZymes, sugar transporters, cytochrome P450, proteases, phosphatases and proteins with other function, were successfully identified. A total of 58 CAZyme proteins were differentially expressed, and at the same time, oxidoreductases participated in lignin degradation were expressed at higher levels in P. eryngii and P. ostreatus. Enzyme activity results indicated that cellulase activities were higher in P. chrysosporium and I. lacteus, while the activities of lignin‐degrading enzymes were higher in P. eryngii and P. ostreatus. In addition to the lignocellulosic degrading enzymes, several proteins including sugar transporters, cytochrome P450 monooxygenases, peptidases, proteinases, phosphatases and kinases were also found to be differentially expressed among these four species of white‐rot fungi. In summary, the protein expression patterns of P. eryngii and P. ostreatus exhibit co‐upregulated oxidoreductase potential and co‐downregulated cellulolytic capability relative to those of P. chrysosporium and I. lacteus, providing a mechanism consistent with selective ligninolysis by P. eryngii and P. ostreatus.
中文翻译：
白腐真菌的比较分泌组揭示了与苎麻茎选择性木质素分解相关的共同调节的碳水化合物活性酶
本研究中， Phanerochaete chrysosporium和Irpex Lacteus同时降解苎麻秆中的木质素和纤维素，而平菇和杏鲍菇只能解聚木质素，但解聚纤维素很少。使用这四种白腐真菌的比较蛋白质组分析来研究这种选择性木质素分解的分子机制。成功鉴定出292个蛋白质，包括CAZymes、糖转运蛋白、细胞色素P450、蛋白酶、磷酸酶和其他功能蛋白。共有58个CAZyme蛋白存在差异表达，同时参与木质素降解的氧化还原酶在杏鲍菇和平菇中表达量较高。酶活性结果表明，黄孢假单胞菌和乳白假单胞菌的纤维素酶活性较高，杏鲍菇和平菇的木质素降解酶活性较高。除了木质纤维素降解酶外，还发现包括糖转运蛋白、细胞色素 P450 单加氧酶、肽酶、蛋白酶、磷酸酶和激酶在内的几种蛋白质在这四种白腐真菌中存在差异表达。总之，相对于金孢子霉和乳糖，杏鲍菇和平菇的蛋白质表达模式表现出共同上调的氧化还原酶潜力和共同下调的纤维素分解能力，这提供了与杏鲍菇选择性木质素分解一致的机制。和P. ostreatus 。
更新日期：2020-08-14
中文翻译：
白腐真菌的比较分泌组揭示了与苎麻茎选择性木质素分解相关的共同调节的碳水化合物活性酶
本研究中， Phanerochaete chrysosporium和Irpex Lacteus同时降解苎麻秆中的木质素和纤维素，而平菇和杏鲍菇只能解聚木质素，但解聚纤维素很少。使用这四种白腐真菌的比较蛋白质组分析来研究这种选择性木质素分解的分子机制。成功鉴定出292个蛋白质，包括CAZymes、糖转运蛋白、细胞色素P450、蛋白酶、磷酸酶和其他功能蛋白。共有58个CAZyme蛋白存在差异表达，同时参与木质素降解的氧化还原酶在杏鲍菇和平菇中表达量较高。酶活性结果表明，黄孢假单胞菌和乳白假单胞菌的纤维素酶活性较高，杏鲍菇和平菇的木质素降解酶活性较高。除了木质纤维素降解酶外，还发现包括糖转运蛋白、细胞色素 P450 单加氧酶、肽酶、蛋白酶、磷酸酶和激酶在内的几种蛋白质在这四种白腐真菌中存在差异表达。总之，相对于金孢子霉和乳糖，杏鲍菇和平菇的蛋白质表达模式表现出共同上调的氧化还原酶潜力和共同下调的纤维素分解能力，这提供了与杏鲍菇选择性木质素分解一致的机制。和P. ostreatus 。
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