Myostatin (MSTN) and myogenic differentiation antigen (MyoD) play an essential role in specification and differentiation of skeletal muscle. However, the role of stress in the regulation of MyoD and MSTN has not been fully revealed and more evidence should be provided. Here, we reported the cloning and expressional analyses of MSTN and MyoD in Large-scale Loach Paramisgurnus dabryanus (hereafter PdMSTN and PdMyoD). Injecting individuals with 0, 60, 600, and 1,200 μg/kg dexamethasone (DXM) for five consecutive days resulted in a dose-dependent change of PdMSTN and PdMyoD expression. The expression of PdMSTN was upregulated with increasing DXM concentrations, while PdMyoD expression was downregulated. The changes in the expression of these genes at different time points for 10 consecutive days were studied after individuals were treated with 600 μg/kg DXM. Compared with the control group, PdMSTN expression decreased and PdMyoD expression increased before 12 h, and both PdMSTN and PdMyoD expression levels increased at 24 h, which was significantly higher than those in control group. At a prolonged treatment of 5-10 d, expression levels of PdMSTN and PdMyoD had significantly reduced. The results indicate that both PdMyoD and PdMSTN are involved in DXM-induced stress in Large-scale Loach.

中文翻译：

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Myostain ( MSTN ) 和肌原性分化抗原 ( MyoD ) 的表征以及地塞米松对其在大型泥鳅 ( Paramisgurnus dabryanus ) 中表达的影响

肌肉生长抑制素 (MSTN) 和成肌分化抗原 (MyoD) 在骨骼肌的规范和分化中起重要作用。然而，压力在MyoD和MSTN调节中的作用尚未完全揭示，应提供更多证据。在这里，我们报告了大型泥鳅 Paramisgurnus dabryanus（以下简称 PdMSTN 和 PdMyoD）中 MSTN 和 MyoD 的克隆和表达分析。连续五天注射 0、60、600 和 1,200 μg/kg 地塞米松 (DXM) 的个体导致 PdMSTN 和 PdMyoD 表达的剂量依赖性变化。PdMSTN 的表达随着 DXM 浓度的增加而上调，而 PdMyoD 的表达则下调。在个体接受 600 μg/kg DXM 治疗后，研究了这些基因在不同时间点连续 10 天的表达变化。与对照组相比，12 h前PdMSTN表达降低，PdMyoD表达升高，24 h后PdMSTN和PdMyoD表达水平均升高，显着高于对照组。在 5-10 天的延长治疗中，PdMSTN 和 PdMyoD 的表达水平显着降低。结果表明，PdMyoD 和 PdMSTN 都参与了 DXM 诱导的大型泥鳅应力。在 5-10 天的延长治疗中，PdMSTN 和 PdMyoD 的表达水平显着降低。结果表明，PdMyoD 和 PdMSTN 都参与了 DXM 诱导的大型泥鳅应力。在 5-10 天的延长治疗中，PdMSTN 和 PdMyoD 的表达水平显着降低。结果表明，PdMyoD 和 PdMSTN 都参与了 DXM 诱导的大型泥鳅应力。