This research has focused on basidiomycete cryopreservation at −80 °C and developed a cryopreservation method based on the use of hard or medium-hard endosperm wheat grains as a mycelial carrier for cryopreservation. The aim of this study was to evaluate the mycelial viability of edible and medicinal basidiomycetes, using 13 strains of Agaricus spp. and eight strains of non-Agaricus spp., cryopreserved at −80 °C on hard endosperm wheat grain, with or without cryoprotectant agent (4% glucose), for two and five years. Two groups of basidiomycetes, Agaricus genus and other non-Agaricus genera, were cryopreserved at −80 °C by wheat grain technique for two and five years. The cryopreservation technique with hard endosperm wheat grain without cryoprotectant (preservation substrate), settled previously for A. subrufescens is efficient to cryopreserve other basidiomycetes such as Lentinus crinitus, Pleurotus ostreatus, Pleurotus eryngii, Schizophyllum commune, and Lentinula edodes, besides A. subrufescens strains.

这项研究集中于-80°C的担子菌冷冻保存，并基于使用硬或中硬胚乳小麦籽粒作为冷冻保存的菌丝体载体，开发了一种冷冻保存方法。这项研究的目的是使用13株姬松茸菌株评估食用和药用担子菌的菌丝活力。和八株非姬松茸菌株，在有或没有冷冻保护剂（4％葡萄糖）的情况下，在-80°C下在硬胚乳小麦籽粒上冷冻保存两年和五年。两组担子菌，姬松茸属和其他非姬松茸属，通过小麦籽粒技术在-80°C下冷冻保存了两年和五年。与硬胚乳小麦的冷冻保存技术而不冷冻保护剂（保护衬底），先前针对定居A. subrufescens是有效的冷冻保存等担子菌如香菇crinitus，平菇，杏鲍菇，裂褶菌，和香菇，除了A. subrufescens菌株。