OBJECTIVE To analyze and compare the expression profile of TAC3, TACR3, KISS1, and KISS1R in mural granulosa and cumulus cells from healthy oocyte donors and patients with different infertility etiologies, including advanced maternal age, endometriosis, and low ovarian response. DESIGN Genetic association study. SETTING Private fertility clinic and public research laboratory. PATIENT(S) Healthy oocyte donors and infertile women undergoing in vitro fertilization (IVF) treatment. INTERVENTION(S) IVF. MAIN OUTCOME MEASURE(S) Gene expression levels of KISS1, KISS1R, TAC3, and TACR3 in human mural granulosa and cumulus cells. RESULT(S) Infertile women showed statistically significantly altered expression levels of KISS1 (-2.57 ± 2.30 vs. -1.37 ± 2.11), TAC3 (-1.21 ± 1.40 vs. -1.49 ± 1.98), and TACR3 (-0.77 ± 1.36 vs. -0.03 ± 0.56) when compared with healthy oocyte donors. Advanced maternal age patients, endometriosis patients, and low responders showed specific and altered expression profiles in comparison with oocyte donors. CONCLUSION(S) Abnormal expression levels of KISS1/KISS1R and TAC3/TACR3 systems in granulosa cells might be involved in the decreased fertility associated to advanced maternal age, endometriosis, and low ovarian response.

中文翻译：

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女性不育与卵巢颗粒细胞和卵丘细胞中神经激肽 B/神经激肽 B 受体和亲吻肽/亲肽素受体系统的表达改变有关

目的 分析比较健康卵母细胞供体和不同病因（包括高龄、子宫内膜异位症和低卵巢反应）的不孕症患者壁颗粒细胞和卵丘细胞中TAC3、TACR3、KISS1和KISS1R的表达谱。设计 遗传关联研究。设置 私人生育诊所和公共研究实验室。患者 接受体外受精 (IVF) 治疗的健康卵母细胞捐献者和不育妇女。干预（S）试管婴儿。主要结果测量 KISS1、KISS1R、TAC3 和 TACR3 在人壁颗粒和卵丘细胞中的基因表达水平。结果（S）不育女性的 KISS1 (-2.57 ± 2.30 vs. -1.37 ± 2.11)、TAC3 (-1.21 ± 1.40 vs. -1.49 ± 1.98) 和 TACR3 (-0.77 ± 1.36 vs. -0.03±0。56) 与健康卵母细胞捐献者相比。与卵母细胞供体相比，高龄产妇、子宫内膜异位症患者和低反应者表现出特异性和改变的表达谱。结论(S)颗粒细胞中KISS1/KISS1R和TAC3/TACR3系统的异常表达水平可能与高龄、子宫内膜异位症和低卵巢反应相关的生育能力下降有关。