First report of basella rugose mosaic virus infecting Anredera cordifolia in mainland of China,Crop Protection

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First report of basella rugose mosaic virus infecting Anredera cordifolia in mainland of China
Crop Protection ( IF 2.5 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.cropro.2020.105350
Zhengnan Li , Pingping Sun , Lei Zhang , Shuang Song

Abstract Anredera cordifolia, in family Basellaceae, is a traditional Chinese medicinal plant and is also widely cultivated as an ornamental vine or a leafy vegetable. In June 2019, A. cordifolia plants displaying symptoms of chlorotic ringspot were observed in Hohhot, Inner Mongolia of China. Flexuous filamentous viral particles of about 800 × 13 nm in size were observed in symptomatic leaf samples. The infection of basella rugose mosaic virus (BaRMV), belonging to the genus Potyvirus, was confirmed by next-generation sequencing of small RNAs and RT-PCR confirmation. Mechanical inoculation assays showed that the BaRMV isolate in this study (BaRMV-IM) can be inoculated to A. cordifolia and Nicotiana rustica, while cannot infect N. benthamiana or N. tabacum. It can't be transmitted by seed. The complete genomes sequence of BaRMV-IM consists of 9862 nt. It shares the highest genome nucleotide sequence identity of 98.5% and polyprotein amino acid sequence identity of 98.6% with those of a BaRMV isolate from Spathiphyllum patinii. Phylogenetic analyses with other potyviruses based on nucleotide and amino acid sequences of polyprotein showed that BaRMV-IM and three other BaRMV isolates available in GenBank clustered to a distinct branch, which was closely related to mirabilis crinkle mosaic virus (MiCMV). No recombination event was detected among the four BaRMV isolates. To our knowledge, this is the first report of occurrence and complete genome sequence of BaRMV in mainland of China.

更新日期：2021-01-01

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