Mesenchymal stem cells have been considered as the suitable source for the repair of kidney lesions. The study and identification of novel approaches could improve the efficiency of these cells in the recovery of kidney. In the present study, the effect of HEK 293 conditioned medium (HEK293-CM) was evaluated on the expression of GDNF/RET signaling pathway and their downstream genes in the human adipose-derived mesenchymal stem cells (AD-MSCs). For this purpose, the human AD-MSCs were cultured in the medium containing HEK293-CM. After the RNA extraction and cDNA synthesis, the expression level of GFRA1, GDNF, SPRY1, ETV4, ETV5, and CRLF1 genes were determined by SYBR Green Real time PCR. The obtained results indicated that the GDNF and GFRA1 expression enhanced in the AD-MSCs following treatment with 10% HEK293-CM-5%FBS as compared to the untreated AD-MSCs. These results were consistent with the decreased expression of SPRY1. The significant increased expression of ETV4, ETV5, and CRLF1 genes also showed that HEK293-CM activated the GDNF/RET signaling pathway in the AD-MSCs (P < 0.05). The obtained data suggested that the treatment with HEK293-CM activated the GDNF/RET signaling pathway in the human AD-MSCs.

间充质干细胞被认为是修复肾脏病变的合适来源。对新方法的研究和鉴定可以提高这些细胞在肾脏恢复中的效率。在本研究中，评估了HEK 293条件培养基（HEK293-CM）对人脂肪来源的间充质干细胞（AD-MSC）中GDNF / RET信号通路及其下游基因表达的影响。为此目的，将人AD-MSC在含有HEK293-CM的培养基中培养。RNA提取和cDNA合成后，通过SYBR Green实时PCR测定GFRA1，GDNF，SPRY1，ETV4，ETV5和CRLF1基因的表达水平。获得的结果表明，与未处理的AD-MSC相比，在用10％HEK293-CM-5％FBS处理后，AD-MSC中的GDNF和GFRA1表达增强。这些结果与SPRY1的表达降低是一致的。ETV4，ETV5和CRLF1基因的表达明显增加也表明，HEK293-CM激活了AD-MSC中的GDNF / RET信号通路（P  <0.05）。获得的数据表明，用HEK293-CM处理激活了人AD-MSC中的GDNF / RET信号通路。