The aim of this work was to study the gene clusters that code for secondary metabolites within the genome of Stemphylium lycopersici and evaluate their toxicity in plant tissue since they are probably responsible for the necrotrophic symptoms provoked by S. lycopersici on plants of the Solanaceae family. We analyzed the draft genome sequences using the antiSMASH software to identify potentially toxic molecules that might be produced by S. lycopersici , and studied the activity of secreted compounds on tomato leaflet and pepper leaves. We found more than 30 gene clusters involved in the synthesis of secondary metabolites. Stemphylium lycopersici secreted compounds that provoked necrosis on tomato leaflets as well as on pepper leaves. In conclusion, S. lycopersici have the potential to produce different secondary metabolites including host-specific and non-host-specific toxins (HST and non-HST) as well as compounds that might confer advantages for their development and survival, under certain environmental conditions. Secondary metabolite gene clusters arrangement and conservation within the genome of Stemphylium lycopersici codes the pathways for the synthesis of specific and non-specific toxins.

中文翻译：

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Stemphylium lycopersici 基因组内的次级代谢产物基因簇的排列和保守性编码了合成特定和非特定毒素的途径

这项工作的目的是研究编码番茄干叶菌基因组中次生代谢物的基因簇，并评估它们在植物组织中的毒性，因为它们可能是由番茄属植物对茄科植物引起的坏死性营养症状的原因。我们使用 antiSMASH 软件分析了基因组序列草图，以确定可能由 S. lycopersici 产生的潜在毒性分子，并研究了番茄小叶和辣椒叶上分泌的化合物的活性。我们发现了 30 多个参与次级代谢产物合成的基因簇。Stemphylium lycopersici 分泌的化合物引起番茄小叶和辣椒叶坏死。总之，S。lycopersici 有可能产生不同的次级代谢产物，包括宿主特异性和非宿主特异性毒素（HST 和非 HST）以及在某些环境条件下可能为其发育和生存带来优势的化合物。Stemphylium lycopersici 基因组内的次级代谢产物基因簇的排列和保守性编码了合成特定和非特定毒素的途径。