Breeding of ornamental marine fishes was initiated in Makassar, South Sulawesi, Indonesia, to protect reef ecosystems from destructive fishing and to supplement income of local communities. However, stress associated with captive breeding conditions could enhance vulnerability of fish to disease. Wild- and captive-bred clownfish Amphiprion percula showed clinical signs such as white spots and warts typically associated with lymphocystis disease. Sequencing a portion of the DNA polymerase gene confirmed the identity of a lymphocystis disease virus (LCDV) in clownfish tissues that closely resembled LCDV from paradisefish; however, both strains were only 90% similar at the nucleotide level. Phylogenetic analysis suggests the LCDV is a new strain representing the first sequence of LCDV in A. percula. To determine the virus presence in clownfish and other ornamental marine species (OMS) in the hatchery, we developed conventional PCR and loop-mediated isothermal amplification (LAMP) assays that were comparatively sensitive at detecting the new LCDV strain. Among the PCR-tested clownfish, 19.4% (n = 14/72) were positive for the LCDV DNA that were either symptomatic or apparently healthy. Other OMS (n = 29) bred in the hatchery including seahorse Hippocampus spp., mandarin fish Synchiropus spp., and pajama cardinal fish Sphaeremia nepatoptera were negative by the LCDV PCR. The PCR and LAMP assays developed in this study will facilitate disease management by early detection of the LCDV and removal of potential reservoirs of the virus.

在印度尼西亚南苏拉威西省望加锡，开始了观赏海洋鱼类的繁殖，以保护珊瑚礁生态系统免受破坏性捕鱼的影响并增加当地社区的收入。但是，与圈养繁殖条件有关的压力可能会增加鱼类对疾病的脆弱性。野生和人工繁殖的小丑鱼两栖动物显示出临床症状，例如通常与淋巴囊肿病相关的白斑和疣。对DNA聚合酶基因的一部分进行测序，证实了小丑鱼组织中淋巴囊肿病病毒（LCDV）的身份，这种病毒与天堂鱼的LCDV非常相似。然而，两种菌株在核苷酸水平上仅相似90％。系统发育分析表明，LCDV是一种新菌株，代表百日草中LCDV的第一个序列。。为了确定孵化场中小丑鱼和其他观赏海洋物种（OMS）中的病毒存在，我们开发了常规PCR和环介导的等温扩增（LAMP）测定法，这些测定法在检测新LCDV株时相对敏感。在经过PCR测试的小丑鱼中， 有症状或看似健康的LCDV DNA阳性率为19.4％（n = 14/72）。其他OMS（Ñ  = 29）饲养在孵化场包括海马海马属，桂鱼连鳍䲗属属，和睡衣基数鱼Sphaeremia nepatopteraLCDV PCR检测为阴性。在这项研究中开发的PCR和LAMP分析将通过早期检测LCDV和清除病毒的潜在库来促进疾病管理。