To investigate the effects of tributyrin supplementation in soybean oil (SO)-based diet, three equal-protein (50%) and equal-lipid (12%) experimental diets were formulated: the fish oil (FO) diet, the SO diet, and the tributyrin (TB) diet with 0.20% tributyrin supplemented in the SO-based diet. Each diet was randomly fed to triplicate tanks with 20 fish (initial weight, 9.00 ± 0.02 g) per tank (300 L). Fish were fed to apparent satiation twice daily for 56 days. The whole body, muscle, and intestine tissue samples were collected for analysis. The results indicated that tributyrin partly reversed the final body weight (TB [39.92 ± 0.33 g] versus SO [35.12 ± 0.55 g]) and feed efficiency rate (TB [0.99 ± 0.01] versus SO [0.92 ± 0.01]) decrease and the whole body crude lipid content increase, caused by the replacement of dietary FO by SO. A significant increase in the polyunsaturated fatty acid proportion was observed in the muscle tissue of fish fed the tributyrin diet. The improvement of growth performance mediated by tributyrin supplementation may be attributed to the significant improvement of the intestinal morphology, as indicated by the morphology structure section and molecular parameters. Dietary tributyrin may decrease the SO-induced pro-inflammatory response, characterized by significantly decreased expression of a pro-inflammatory gene (IL-6) and significantly increased expression of an anti-inflammatory gene (IL-10) (P < 0.05). In conclusion, dietary 0.20% tributyrin supplementation in the SO-based diet improved growth performance, affected muscle fatty acid composition, and improved intestinal histology structure in the juvenile yellow drum.

为了研究补充三丁酸甘油酯在以豆油（SO）为基础的日粮中的作用，配制了三种等蛋白（50％）和等脂（12％）实验日粮：鱼油（FO）日粮，SO日粮，以及以SO为基础的饮食中补充了0.20％的三丁酸甘油三酯（TB）饮食。将每种饮食随机喂入一式三份的水箱中，每箱（300升）有20条鱼（初始重量，9.00±0.02克）。每天两次对鱼进行明显的饱食，持续56天。收集全身，肌肉和肠组织样本进行分析。结果表明，三丁酸甘油酯可部分逆转最终体重（TB [39.92±0.33 g]相对于SO [35.12±0.55 g]），饲料利用率（TB [0.99±0.01]相对SO [0.92±0.01]）降低，且SO替代日粮FO导致全身粗脂质含量增加。在饲喂三丁精饮食的鱼的肌肉组织中观察到多不饱和脂肪酸比例的显着增加。如形态结构部分和分子参数所示，补充三丁酸甘油酯所介导的生长性能的改善可能归因于肠道形态的显着改善。膳食三丁酸甘油酯可能会降低SO诱导的促炎反应，其特征在于促炎基因（IL-6）的表达显着降低，而抗炎基因（IL-10）的表达显着增加（如形态结构部分和分子参数所示，补充三丁酸甘油酯所介导的生长性能的改善可归因于肠道形态的显着改善。膳食三丁酸甘油酯可能会降低SO诱导的促炎反应，其特征在于促炎基因（IL-6）的表达显着降低，而抗炎基因（IL-10）的表达显着增加（如形态结构部分和分子参数所示，补充三丁酸甘油酯所介导的生长性能的改善可能归因于肠道形态的显着改善。膳食三丁酸甘油酯可能会降低SO诱导的促炎反应，其特征在于促炎基因（IL-6）的表达显着降低，而抗炎基因（IL-10）的表达显着增加（P  <0.05）。总之，以SO为基础的饮食中添加0.20％的三丁酸甘油酯可改善幼年黄鼓中的生长性能，影响肌肉脂肪酸组成并改善肠道组织学结构。