Psoriasis vulgaris (PV) is a chronic autoimmune inflammatory disease with epidermal hyperkeratosis and parakeratosis. The study was to elucidate the pathogenesis of PV by quantitative proteomic analysis of skin lesion biopsies of PV and healthy tissues with tandem mass tags (TMTs) coupled with liquid chromatography–mass spectrometry (LC–MS)/MS. A total of 4562 differentially expressed proteins (DEPs) between PV lesional tissues (n = 11) and healthy tissues (n = 11) were identified, of which 299 were upregulated and 206 were downregulated using |fold change| > 1.3 as the cutoff threshold. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the DEPs were mainly enriched in the activation of immune cells (drug metabolism pathway, NOD-like pathway, and IL-17 pathway), cell proliferation (ribosomal pathway, DNA replication pathway, and base replication pathway), metabolism-related pathways (fatty acid biosynthesis and metabolism, PPAR pathway, glycerophospholipid metabolism, and cortisol synthesis and breakdown), and glandular secretion (saliva secretion, gastric acid secretion, and pancreatic fluid secretion). Thirteen DEPs that were relatively highly expressed in the drug metabolism pathway were validated with parallel reaction monitoring (PRM), of which MPO, TYMP, IMPDH2, GSTM4, and ALDH3A1 were highly expressed in PV, whereas CES1, MAOB, MGST1, and GSTT1 were less expressed in PV. These findings confirmed that these proteins participate in the drug metabolism-other enzyme pathways and play crucial roles in the activation and proliferation of immune cells in the pathogenesis of PV.

中文翻译：

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使用串联质量标签和平行反应监测定量分析寻常型银屑病中差异表达的蛋白质。

寻常型银屑病 (PV) 是一种慢性自身免疫性炎症性疾病，伴有表皮角化过度和角化不全。该研究旨在通过串联质谱标签 (TMT) 结合液相色谱-质谱 (LC-MS)/MS 对 PV 和健康组织的皮肤病变活检进行定量蛋白质组学分析来阐明 PV 的发病机制。在 PV 病变组织 (n = 11) 和健康组织 (n = 11) 之间共鉴定出 4562 种差异表达蛋白 (DEP)，其中 299 种上调，206 种下调使用|倍数变化| > 1.3 作为截止阈值。基因本体论（GO）注释和京都基因与基因组百科全书（KEGG）通路富集分析表明，DEPs主要富集于免疫细胞的激活（药物代谢通路、NOD样通路和IL-17通路），细胞增殖（核糖体途径、DNA复制途径和碱基复制途径）、代谢相关途径（脂肪酸生物合成和代谢、PPAR途径、甘油磷脂代谢、皮质醇合成和分解）和腺体分泌（唾液分泌、胃酸分泌）和胰液分泌）。通过平行反应监测 (PRM) 验证了 13 个在药物代谢途径中相对高表达的 DEP，其中 MPO、TYMP、IMPDH2、GSTM4 和 ALDH3A1 在 PV 中高表达，而 CES1、MAOB、MGST1 和 GSTT1 在 PV 中高表达。 PV 表示较少。这些发现证实，这些蛋白质参与药物代谢-其他酶途径，并在 PV 发病机制中免疫细胞的激活和增殖中起关键作用。