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Aptamer-enhanced fluorescence determination of bisphenol A after magnetic solid-phase extraction using Fe3O4@SiO2@aptamer.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-14 , DOI: 10.1039/d0ay01124j Zhou Xu 1 , Yanqiu Chen , Yao Tang , Maolong Chen , Wei Chen , Yunhui Cheng
Analytical Methods ( IF 2.7 ) Pub Date : 2020-08-14 , DOI: 10.1039/d0ay01124j Zhou Xu 1 , Yanqiu Chen , Yao Tang , Maolong Chen , Wei Chen , Yunhui Cheng
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Bisphenol A (BPA) is used as a stabilizing agent in many food packaging plastics and is a known endocrine-disrupting chemical that can alter the development of mammary glands, affect egg cells, and cause chromosomal defects. However, the pretreatment of traditional assays for detecting BPA is difficult. In this work, a novel aptamer functionalized magnetic adsorbent was developed and combined with magnetic solid-phase extraction (MSPE) for the selective enrichment of BPA. First, magnetic silica-coated Fe3O4 microspheres (Fe3O4@SiO2) were synthesized by the sol–gel method, and functional magnetic nanoparticles (Fe3O4@SiO2@Apt) were formed by modifying with nucleic acids. In the presence of BPA in a MSPE system, the nucleic acid aptamer can specifically capture the target BPA. After magnetic separation, the Apt/BPA composite was eluted, and we observed enhanced fluorescence with the Apt/BPA composite that was formed. Our results showed that this method allowed a limit of detection of 0.05 ng mL−1.
中文翻译：
磁性固相萃取后使用Fe3O4 @ SiO2 @ aptamer进行适体增强的荧光测定双酚A。
双酚A（BPA）在许多食品包装塑料中用作稳定剂，并且是一种已知的破坏内分泌的化学物质，可以改变乳腺的发育，影响卵细胞并引起染色体缺陷。然而，用于检测BPA的传统测定的预处理是困难的。在这项工作中，开发了一种新型的适体功能化磁性吸附剂，并与磁性固相萃取（MSPE）结合用于选择性富集BPA。首先，通过溶胶-凝胶法合成了磁性二氧化硅包覆的Fe 3 O 4微球（Fe 3 O 4 @SiO 2）和功能性磁性纳米粒子（Fe 3 O 4 @SiO 2）。通过用核酸修饰而形成。在MSPE系统中存在BPA的情况下，核酸适体可以特异性捕获靶BPA。磁分离后，洗脱出Apt / BPA复合物，并且观察到形成的Apt / BPA复合物的荧光增强。我们的结果表明，该方法的检出限为0.05 ng mL -1。
更新日期：2020-09-24
中文翻译：
磁性固相萃取后使用Fe3O4 @ SiO2 @ aptamer进行适体增强的荧光测定双酚A。
双酚A（BPA）在许多食品包装塑料中用作稳定剂，并且是一种已知的破坏内分泌的化学物质，可以改变乳腺的发育，影响卵细胞并引起染色体缺陷。然而，用于检测BPA的传统测定的预处理是困难的。在这项工作中，开发了一种新型的适体功能化磁性吸附剂，并与磁性固相萃取（MSPE）结合用于选择性富集BPA。首先，通过溶胶-凝胶法合成了磁性二氧化硅包覆的Fe 3 O 4微球（Fe 3 O 4 @SiO 2）和功能性磁性纳米粒子（Fe 3 O 4 @SiO 2）。通过用核酸修饰而形成。在MSPE系统中存在BPA的情况下，核酸适体可以特异性捕获靶BPA。磁分离后，洗脱出Apt / BPA复合物，并且观察到形成的Apt / BPA复合物的荧光增强。我们的结果表明，该方法的检出限为0.05 ng mL -1。
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