The development of a microplatform with multifunctional integration allowing the dynamic and high-throughput exploration of three-dimensional (3D) cultures is promising for biomedical research. Here, we introduce an integrated microfluidic 3D tumor system with pneumatic manipulation and chemical gradient generation to investigate anticancer therapy in a parallel, controllable, dynamic, and high-throughput manner. The stability of the microfluidic system to realize precise and long-term chemical gradient production was developed. Serial manipulations including active cell trapping, array-like tumor self-assembly and formation, reliable gradient generation, parallel multi-concentration drug stimulation, and real-time tumor analysis were achieved in a single microfluidic device. The microfluidic platform was demonstrated to be stable for high-throughput cell trapping and 3D tumor formation with uniform quantities. On-chip analysis of phenotypic tumor responses to diverse chemotherapies with different concentrations can be conducted in this device. The microfluidic advancement holds great potential for applications in the development of high-performance and multi-functional biomimetic tumor systems and in the fields of cancer research and pharmaceutical development.

中文翻译：

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用于并行和高通量化疗评估的集成微流控3D肿瘤系统

具有多功能集成的微平台的开发允许对三维（3D）文化进行动态和高通量探索，这对生物医学研究很有前途。在这里，我们介绍了一种集成的微流体3D肿瘤系统，该系统具有气动操作和化学梯度生成功能，以并行，可控制，动态和高通量的方式研究抗癌治疗。开发了用于实现精确和长期化学梯度产生的微流体系统的稳定性。在单个微流控设备中实现了包括活细胞捕获，阵列状肿瘤自组装和形成，可靠的梯度产生，平行多浓度药物刺激以及实时肿瘤分析在内的一系列操作。事实证明，微流体平台对于高通量细胞捕获和数量均匀的3D肿瘤形成是稳定的。可以在该设备中对不同浓度的多种化学疗法的表型肿瘤反应进行芯片分析。微流体的发展在高性能和多功能仿生肿瘤系统的开发以及癌症研究和药物开发领域中具有巨大的应用潜力。