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Evaluation of swabs from 15 commercially available oral fluid sample collection devices for the analysis of commonly abused substances: doping agents and drugs of abuse
Analyst ( IF 3.6 ) Pub Date : 2020-08-13 , DOI: 10.1039/d0an01379j Łukasz Sobczak 1, 2, 3, 4, 5 , Krzysztof Goryński 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2020-08-13 , DOI: 10.1039/d0an01379j Łukasz Sobczak 1, 2, 3, 4, 5 , Krzysztof Goryński 1, 2, 3, 4, 5
Affiliation
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Oral fluid testing is steadily building its position as a valuable complement or alternative to plasma and urine analyses in everyday laboratory practice. However, the great significance of the sample collection process in the attainment of representative results is not always paralleled by the attention given to its informed selection. Few evaluations of commercially available sample collection devices have been published until now, and the current work intends to fill this gap by presenting an evaluation of swabs from 15 different devices for the analysis of 49 popular drugs. Swabs, derived from sample collection devices, were used to collect a drug-fortified mixture. Then, swab-retrieved samples were subjected to instrumental analysis with the high-performance liquid chromatography coupled with tandem mass spectrometry method. Results within the 80–120% range were considered to have no significant impact on analyte concentration (thus satisfactory) and were observed in 44.1% of all results. Out of the 15 evaluated swabs, 7 provided results in the aforementioned range for more than half of the substances under study. The possibility of matrix effects originating from swab materials was also investigated. The selection of an appropriate oral fluid sample collection method plays a critical role in the success of the analytical procedure, a fact that is well-illustrated by the tremendous differences between analyte concentrations observed in this research. Perhaps, the tedious labour of improving sample preparation and analysis methods already in-use could be spared if only greater emphasis were to be put on the improvement and better selection of suitable solutions for oral fluid collection.
中文翻译：
评估15种市售口腔液样本采集设备中的拭子，以分析常见滥用物质：掺杂剂和滥用药物
口服液测试正在稳步确立其在日常实验室实践中作为血浆和尿液分析的宝贵补充或替代品的地位。但是，样品采集过程对获得代表性结果的重要意义并不总是与对样品知情选择的关注并驾齐驱。迄今为止，几乎没有对商用样品采集设备进行评估，目前的工作旨在通过对15种不同设备的拭子进行评估来填补这一空白，以分析49种流行药物。从样品收集装置中提取的拭子用于收集药物强化的混合物。然后，用高效液相色谱结合串联质谱法对拭子回收的样品进行仪器分析。80-120％范围内的结果被认为对分析物浓度没有显着影响（因此令人满意），在所有结果中有44.1％观察到。在15个评估棉签中，有7个提供了上述范围内一半以上所研究物质的结果。还研究了源自拭子材料的基质效应的可能性。选择适当的口腔液样品收集方法在分析程序的成功中起着至关重要的作用，这一事实在本研究中观察到的分析物浓度之间的巨大差异就充分说明了这一事实。也许，
更新日期：2020-09-05
中文翻译：
评估15种市售口腔液样本采集设备中的拭子，以分析常见滥用物质：掺杂剂和滥用药物
口服液测试正在稳步确立其在日常实验室实践中作为血浆和尿液分析的宝贵补充或替代品的地位。但是，样品采集过程对获得代表性结果的重要意义并不总是与对样品知情选择的关注并驾齐驱。迄今为止，几乎没有对商用样品采集设备进行评估，目前的工作旨在通过对15种不同设备的拭子进行评估来填补这一空白，以分析49种流行药物。从样品收集装置中提取的拭子用于收集药物强化的混合物。然后，用高效液相色谱结合串联质谱法对拭子回收的样品进行仪器分析。80-120％范围内的结果被认为对分析物浓度没有显着影响（因此令人满意），在所有结果中有44.1％观察到。在15个评估棉签中，有7个提供了上述范围内一半以上所研究物质的结果。还研究了源自拭子材料的基质效应的可能性。选择适当的口腔液样品收集方法在分析程序的成功中起着至关重要的作用，这一事实在本研究中观察到的分析物浓度之间的巨大差异就充分说明了这一事实。也许，
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