Objective: Molecularly Imprinted Polymer (MIP)-modified potentiometric sensors for histamine (HIS) (as denoted as HIS sensor) have been developed.

Methods: The MIPs comprise HIS, Methacrylic Acid (MAA) and ethylene glycol dimethacrylate as the template molecule, functional monomer and cross-linker, respectively. To examine the specificity of the MIP to HIS, the MIP particles were prepared with varying ratios of HIS: MAA and the HIS binding amount toward the MIP particles was determined by UV spectrophotometry. Furthermore, to quantitatively determine the ability of MIP (H2M20) to HIS, a HIS sensor was measured using Ag/AgCl as a reference electrode.

Results: MIP particles having a HIS:MAA of 2 mmol:20 mmol (MIP (H2M20)) had the largest HIS binding amount among the MIP particles prepared. Additionally, MIP (H2M20) displayed a HIS binding amount approximately two times larger than the corresponding non-imprinted polymer (NIP) particles in the absence of template. The HIS sensor potential change increased as a function of HIS concentration and exhibited a near-Nernstian response of −25.7 mV decade−1 over the HIS concentration range of 1×10−5 to 1×10−4 mol L−1 with a limit of detection of 9.6×10−6 mol L−1. From the Nernstian response value, it was observed that the HIS sensor could detect the di-protonated HIS binding to the MIP. Conversely, when comparing at the same HIS concentration, the potential response value of the sensors fabricated using NIP particles were significantly smaller than the values of the corresponding HIS sensor.

Conclusion: The MIP-modified potentiometric sensors can potentially be employed as an analytical method to quantitatively determine various analytes.

目的：已开发出分子印迹聚合物（MIP）修饰的组胺电位传感器（HIS）（称为HIS传感器）。

方法：MIP分别以HIS，甲基丙烯酸（MAA）和乙二醇二甲基丙烯酸酯为模板分子，功能单体和交联剂。为了检查MIP对HIS的特异性，制备了具有不同比率的HIS：MAA的MIP颗粒，并通过紫外分光光度法确定了HIS对MIP颗粒的结合量。此外，为了定量确定MIP（H2M20）对HIS的能力，使用Ag / AgCl作为参比电极测量了HIS传感器。

结果：在制备的MIP颗粒中，HIS：MAA为2 mmol：20 mmol的MIP颗粒（MIP（H2M20））具有最大的HIS结合量。此外，在没有模板的情况下，MIP（H2M20）的HIS结合量约为相应的非压印聚合物（NIP）颗粒的两倍。HIS传感器的电势变化随HIS浓度的增加而增加，并且在1×10-5至1×10-4 mol L-1的HIS浓度范围内显示出-25.7 mV十进制-1的近能谱响应9.6×10-6 mol L-1的检测。从能斯特响应值，观察到HIS传感器可以检测到双质子化的HIS与MIP的结合。相反，在相同的HIS浓度下进行比较时，

结论：MIP修饰的电位传感器可潜在地用作定量确定各种分析物的分析方法。