Summary The Draize rabbit eye irritation test has several disadvantages and inadequacies when used as an indicator for potential irritancy of detergent-based commercial products. As a possible in vitro screen, it was decided to use mouse LS cells cultured in suspension, taking 50% cell death (CD50) after exposure to the product for 4h as the endpoint. This figure for 11 formulations was compared with eye irritation data ranked using an arbitrary classification of mild, moderate and severe. All samples with a CD50 less than 0.5mg/ml were severe eye irritants, while most of those with a CD50 greater than 1.0mg/ml were mild irritants. Samples between these two cytotoxicity levels were, in general, moderately irritant to the rabbit eye. It would appear that this in vitro test is a possible screen for the irritancy of detergent-based products.

中文翻译：

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基于洗涤剂的商业产品对悬浮培养小鼠系细胞的毒性作为 Draize 兔眼刺激试验的可能筛选

总结 当用作基于洗涤剂的商业产品潜在刺激性的指标时，Draize 兔眼刺激试验有几个缺点和不足之处。作为一种可能的体外筛选，决定使用悬浮培养的小鼠 LS 细胞，以接触产品 4 小时后 50% 的细胞死亡 (CD50) 作为终点。将 11 种配方的这个数字与使用轻度、中度和重度的任意分类分级的眼睛刺激数据进行比较。CD50 小于 0.5mg/ml 的所有样品都是严重的眼刺激物，而 CD50 大于 1.0mg/ml 的大多数样品是轻度刺激物。这两种细胞毒性水平之间的样品通常对兔眼有中等刺激性。看来，这种体外测试可能是对基于洗涤剂的产品刺激性的筛选。