All eukaryotic cells rely on endocytosis to regulate the plasma membrane proteome and lipidome. Most eukaryotic groups, with the exception of fungi and animals, have retained the evolutionary ancient TSET complex as a regulator of endocytosis. Despite the presence of similar building blocks in TSET, compared to other coatomer complexes, structural insight into this adaptor complex is lacking. Here, we elucidate the molecular architecture of the octameric plant TSET complex (TPLATE complex/TPC) using an integrative structural approach. This allowed us to describe a plant-specific connection between the TML subunit and the AtEH/Pan1 proteins and show a direct interaction between the complex and the plasma membrane without the need for any additional protein factors. Furthermore, we identify the appendage of TPLATE as crucial for complex assembly. Structural elucidation of this ancient adaptor complex vastly advances our functional as well as evolutionary insight into the process of endocytosis.

中文翻译：

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TPLATE亚基对于内吞TSET复合物的结构组装至关重要

所有的真核细胞都依赖内吞作用来调节质膜蛋白质组和脂质组。除真菌和动物外，大多数真核生物都保留了进化的古老TSET复合物作为内吞作用的调节剂。尽管与其他涂料复合物相比，TSET中存在类似的构建基块，但仍缺乏对该衔接子复合物的结构了解。在这里，我们使用整合结构方法阐明了八聚体植物TSET复合物（TPLATE复合物/ TPC）的分子结构。这使我们能够描述TML亚基与AtEH / Pan1蛋白之间的植物特异性连接，并显示了复合物与质膜之间的直接相互作用，而无需任何其他蛋白质因子。此外，我们认为TPLATE的附件对于复杂的组装至关重要。