Abstract

Purpose

To study the environmental radiation effects of wild animals after the Fukushima Dai-ichi nuclear power plant accident, we assessed effects on hematopoietic progenitor cells (HPCs) in large Japanese field mice (Apodemus speciosus).

Materials and methods

A. speciosus were collected from three contaminated sites and control area. The air dose-rates at the control and contaminated areas were 0.96 ± 0.05 μGy/d (Hirosaki), 14.4 ± 2.4 μGy/d (Tanashio), 208.8 ± 31.2 μGy/d (Ide), 470.4 ± 93.6 μGy/d (Omaru), respectively. We investigated possible DNA damage and pro-inflammatory markers in the bone marrow (BM) cells. The colony-forming potential of BM cells was estimated by the number of HPC colony-forming cells. Radiation-induced genomic instability (RIGI) in HPCs was also analyzed by quantifying delayed DNA damage in CFU-GM clones.

Results

Although no significant differences in DNA damage and inflammation markers in BM cells from control and contaminated areas, the number of HPC colonies exhibited an inverse correlation with air dose-rate. With regard to RIGI, no significant differences in DNA damage of CFU-GM clones between the mice from the control and the three contaminated areas.

Conclusions

Our study suggests that low dose-rate radiation of more than 200 Gy/d reduced HPCs, possibly eliminating genomically unstable HPCs.

中文翻译：

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福岛大型日本田间小鼠的环境辐射降低了造血祖细胞的集落形成潜力，而不会导致基因组不稳定性

摘要

目的

为了研究福岛第一核电站事故后野生动物对环境辐射的影响，我们评估了对大型日本田鼠 ( Apodemus speciosus ) 造血祖细胞 (HPC) 的影响。

材料和方法

A. speciosus 是从三个污染地点和控制区收集的。控制区和污染区的空气剂量率为 0.96 ± 0.05 μGy/d（Hirosaki）、14.4 ± 2.4 μGy/d（Tanashio）、208.8 ± 31.2 μGy/d（Ide）、470.4 ± 93.6 μGy/d（Omaru ）， 分别。我们调查了骨髓 (BM) 细胞中可能的 DNA 损伤和促炎标志物。BM 细胞的集落形成潜力通过 HPC 集落形成细胞的数量来估计。还通过量化 CFU-GM 克隆中的延迟 DNA 损伤来分析 HPC 中辐射诱导的基因组不稳定性 (RIGI)。

结果

尽管来自对照和污染区域的 BM 细胞中的 DNA 损伤和炎症标志物没有显着差异，但 HPC 菌落的数量与空气剂量率呈负相关。关于 RIGI，CFU-GM 克隆的 DNA 损伤在对照组和三个污染区域的小鼠之间没有显着差异。

结论

我们的研究表明，超过 200 Gy/d 的低剂量率辐射降低了 HPC，可能消除了基因组不稳定的 HPC。