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Genetic diversity and variability among pigmented rice germplasm using molecular marker and morphological traits
Biotechnology & Biotechnological Equipment ( IF 1.5 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1804451
Hamidah Mohd Sarif 1 , Mohd Y. Rafii 1, 2 , Asfaliza Ramli 3 , Yusuff Oladosu 2 , Hanafi M. Musa 2 , Harun A. Rahim 4 , Zakiah Mohd Zuki 1 , Samuel Chibuike Chukwu 2
Affiliation  

Abstract Exploring the genetic aspects of germplasm using genotypic and phenotypic analysis is more reliable for the development of rice varieties that could lead to the attainment of food security. This is because genetic variations provide a space for recombinants, which is an important factor in new varietal development. This study was conducted to evaluate genetic variability and diversity among 32 coloured rice accessions using agro-morphological characteristics and simple sequence repeats (SSR) markers. Quantitative traits (morphological, grain quality and antioxidant properties) and 34 SSR molecular markers were used as tools for determining cultivar identities and genetic diversity. Most of the quantitative traits showed significant differences (p ≤ 0.01) among all rice accessions. Clustering analysis from quantitative traits categorised the accessions into four groups. Similarly, the 32 accessions were grouped into 4 cluster groups based on the analysis of 34 SSR markers. The accessions YTM15, Pulut Merah 3, Padi Randau, Ringan Bawang, DNJ128 and DV 107 can be potentially selected for development of new varieties for local cultivation. Finally, these accessions can be used as parents in further breeding programmes.

中文翻译:

利用分子标记和形态性状研究有色水稻种质的遗传多样性和变异性

摘要 使用基因型和表型分析探索种质的遗传方面对于开发可实现粮食安全的水稻品种更为可靠。这是因为遗传变异为重组体提供了空间,这是新品种开发的重要因素。本研究旨在利用农业形态特征和简单序列重复 (SSR) 标记评估 32 个有色水稻品种之间的遗传变异性和多样性。数量性状(形态、谷物品质和抗氧化特性)和 34 个 SSR 分子标记被用作确定品种身份和遗传多样性的工具。在所有水稻种质中,大多数数量性状表现出显着差异(p ≤ 0.01)。从数量性状的聚类分析将种质分为四组。同样,根据对 34 个 SSR 标记的分析,将 32 个种质分为 4 个聚类组。可以选择 YTM15、Pulut Merah 3、Padi Randau、Ringan Bawang、DNJ128 和 DV 107 种质用于开发本地栽培的新品种。最后,这些种质可用作进一步育种计划的亲本。
更新日期:2020-01-01
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