ABSTRACT

RNA editing is an essential process for modifying nucleotides at specific RNA sites during post-transcription in many species. However, its genomic landscape and characters have not been systematically explored in the bovine genome. In the present study, we characterized global RNA editing profiles from 50 samples of cattle and revealed a range of RNA editing profiles in different tissues. Most editing sites were significantly enriched in specific BovB-derived SINEs, especially the dispersed Bov-tAs, which likely forms dsRNA structures similar to the primate-specific Alu elements. Interestingly, ADARB1 (ADAR2) was observed to be predominant in determining global editing in the bovine genome. Common RNA editing sites among similar tissues were associated with tissue-specific biological functions. Taken together, the wide distribution of RNA editing sites and their tissue-specific characters implied the bovine RNA editome should be further explored.

摘要

在许多物种的转录后过程中，RNA 编辑是在特定 RNA 位点修饰核苷酸的重要过程。然而，尚未在牛基因组中系统地探索其基因组景观和特征。在本研究中，我们表征了来自 50 个牛样本的全局 RNA 编辑谱，并揭示了不同组织中的一系列 RNA 编辑谱。大多数编辑位点显着富含特定的 BovB 衍生的 SINE，尤其是分散的 Bov-tAs，它可能形成类似于灵长类动物特异性 Alu 元件的 dsRNA 结构。有趣的是，观察到 ADARB1 (ADAR2) 在确定牛基因组中的全局编辑方面起主导作用。相似组织中常见的 RNA 编辑位点与组织特异性生物学功能有关。综合起来，