ABSTRACT

Although skin infections are usually uncomplicated, it may indicate systemic disease or lead to life-threatening systemic infections. Staphylococcus aureus is the most common cause of skin infections. Treatment is complicated by continuous emergence of resistant S. aureus especially methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA). Therefore, searching for new alternatives is essential to combat this escalating antibiotic resistance. Bacteriophage therapy is a promising solution in the post antibiotic area. Bacteriophage therapy confers safety and higher rate of success. In the present study, 10 bacteriophages (belonging to the family Siphoviridae and Myoviridae) were isolated with lytic activity against MRSA &VRSA isolated from skin infections. The strongest six of ten phages were characterized morphologically by a JOEL_JEM_1010 electron microscope, physically through evaluating their lytic activity against wide ranges of temperature (35–95° C), PH (3–10) and radiation doses (0.5–2.5kGy) and biologically by evaluating their host ranges and phage longevity (1–120 day) in vitro. Finally, the anti-Staphylococcal effect of the isolated bacteriophages was tested and revealed that they have alone activity (10, 12) and synergistic activity with vancomycin and erythromycin by increasing their inhibition zone diameters (16, 22) mm, respectively.

抽象的

尽管皮肤感染通常并不复杂，但它可能表示全身性疾病或导致威胁生命的全身性感染。金黄色葡萄球菌是皮肤感染的最常见原因。耐药性金黄色葡萄球菌特别是耐甲氧西林的金黄色葡萄球菌（MRSA）和耐万古霉素的金黄色葡萄球菌（VRSA）的不断出现使治疗变得复杂。因此，寻找新的替代品对于对抗这种不断升级的抗生素耐药性至关重要。在后抗生素领域，噬菌体疗法是​​一种有前途的解决方案。噬菌体疗法可确保安全性和更高的成功率。在本研究中，有10个噬菌体（属于Siphoviridae和分离出具有对从皮肤感染分离的MRSA和VRSA具有裂解活性的肌病毒科。用JOEL_JEM_1010电子显微镜对十个噬菌体中最强的六个进行形态学表征，通过评估它们在宽温度（35–95°C），PH（3–10）和辐射剂量（0.5–2.5kGy）和通过在体外评估其宿主范围和噬菌体寿命（1-120天）进行生物学研究。最后，测试了分离的噬菌体的抗葡萄球菌作用，发现它们分别具有单独的活性（10、12）和与万古霉素和红霉素的协同活性，分别通过增加其抑菌圈直径（16、22）mm。