Lyme disease and anaplasmosis are tick-borne bacterial diseases caused by Borreliella and Anaplasma species, respectively. A comprehensive analysis of the exposure of eastern coyotes (Canis latrans) in the northeastern United States to tick-borne pathogens has not been conducted. In this report, we assess the serological status of 128 eastern coyotes harvested in Pennsylvania in 2015 and 2017 for antibodies to Borreliella burgdorferi and Anaplasma phagocytophilum. Immunoblot and dot blot approaches were employed to test each plasma sample by using cell lysates and recombinant proteins as detection antigens. The results demonstrate high seropositivity incidences of 64.8% and 72.7% for B. burgdorferi and A. phagocytophilum, respectively. Antibodies to both pathogens were detected in 51.5% of the plasma samples, indicating high potential for coinfection. Antibodies to the B. burgdorferi proteins DbpB, VlsE, DbpA, BBA36, and OspF (BBO39) were detected in 67.2, 63.3, 56.2, 51.6, and 48.4% of the plasma samples, respectively. Antibodies to the A. phagocytophilum P44 and P130 proteins were detected in 72.7 and 60.9% of the plasma samples, respectively.

中文翻译：

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宾夕法尼亚州东部郊狼（Canis latrans）接触蜱传病原体伯氏疏螺旋体和嗜噬细胞无形体的血清学证据。

莱姆病和无形体病分别是由疏螺旋体和无形体物种引起的蜱传细菌性疾病。尚未对美国东北部东部郊狼 ( Canis latrans )接触蜱传病原体的情况进行综合分析。在本报告中，我们评估了 2015 年和 2017 年在宾夕法尼亚州收获的 128 只东部郊狼的血清学状态，以检测伯氏疏螺旋体和嗜吞噬细胞无形体的抗体。通过使用细胞裂解物和重组蛋白作为检测抗原，采用免疫印迹和斑点印迹方法来测试每个血浆样品。结果表明，高血清阳性率分别为 64.8% 和 72.7%B. burgdorferi和A. phagocytophilum，分别。在 51.5% 的血浆样本中检测到针对这两种病原体的抗体，这表明合并感染的可能性很高。抗体与博氏疏螺旋，分别63.3 56.2，51.6，和血浆样品的48.4％，在67.2检测到的蛋白质DbpB，VlsE，DBPA，BBA36和OSPF（BBO39）。分别在 72.7% 和 60.9% 的血浆样品中检测到A. phagocytophilum P44 和 P130 蛋白的抗体。