The time course response of selected phytohormones has been evaluated in sweet pepper plants (Capsicum annuum L.) submitted to a high density (200 aphids/plant) of aphid (Myzus persicae Sulzer) infestation. Abscisic acid (ABA), salicylic acid (SA), indole-3-acetic acid (IAA), and jasmonates (JAs), including jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and cis-OPDA have been simultaneously identified and quantitated by UHPLC-MS/MS in pepper leaf tissue harvested at 3, 8 hours post-infestation (hpi), 1, 2, 4 and 7 days post-infestation (dpi). Infested plants showed a reduction in stem length at 7 dpi and in the number of leaves and leaf width from 4 dpi onwards. JA and JA-Ile significantly increased very early (from 3 hpi) while SA only accumulated at 7 dpi. Despite the high density of infestation, the aphid-induced accumulation of JAs was much lower than the burst typically induced by chewing herbivores. On the other side, ABA peaked in aphid-infested plants at 2 and 4 dpi, while IAA content did not change significantly at any time point. Growth inhibition may be partially explained by the high levels of JAs found in aphid-infested plants. The possibility that the obtained results support the hypothesis of the aphid manipulation of plant metabolism is discussed. This article is protected by copyright. All rights reserved.

中文翻译：

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高密度蚜虫侵染下辣椒（ Capsicum annuum L. ）叶片中的植物激素反应

已在遭受高密度（200 只蚜虫/植物）蚜虫（Myzus persicae Sulzer）侵染的甜椒植物（Capsicum annuum L.）中评估了所选植物激素的时间进程响应。脱落酸 (ABA)、水杨酸 (SA)、吲哚-3-乙酸 (IAA) 和茉莉酸酯 (JA)，包括茉莉酸 (JA)、茉莉酰-L-异亮氨酸 (JA-Ile) 和顺式 OPDA已通过 UHPLC-MS/MS 在侵染后 3、8 小时 (hpi)、侵染后 1、2、4 和 7 天 (dpi) 收获的辣椒叶组织中同时进行鉴定和定量。受侵染的植物在 7 dpi 时茎长减少，从 4 dpi 开始叶数和叶宽减少。JA 和 JA-Ile 很早就显着增加（从 3 hpi 开始），而 SA 仅在 7 dpi 时积累。尽管感染率很高，蚜虫诱导的 JAs 积累远低于通常由咀嚼食草动物引起的爆发。另一方面，ABA 在蚜虫侵染的植物中在 2 dpi 和 4 dpi 达到峰值，而 IAA 含量在任何时间点都没有显着变化。在蚜虫侵染的植物中发现的高水平 JA 可能部分解释了生长抑制。讨论了所得结果支持蚜虫操纵植物代谢假说的可能性。本文受版权保护。版权所有。讨论了所得结果支持蚜虫操纵植物代谢假说的可能性。本文受版权保护。版权所有。讨论了所得结果支持蚜虫操纵植物代谢假说的可能性。本文受版权保护。版权所有。