In this study, we aimed to assess whether the composite of chitosan/ZnO‐doped bioglass can be applied as a suitable scaffold for the incorporation of bioactive peptides. Material of a porous composite with 1:1 ratio of bioglass:polymer was produced and used as a matrix for delivery of peptide. A peptide with the PEPTIDES sequence (Pro‐Glu‐Pro‐Thr‐Ile‐Asp‐Glu‐Ser) was chosen as a model peptide. Microstructure and pore sizes of chitosan/ZnO‐doped bioglass were assessed. Open porosity and pore sizes of the composite were suitable for enabling the migration of cells and ensuring the easy delivery of nutrients within the implant. In addition, composite showed bioactivity and bactericidal activity against Staphylococcus aureus and Pseudomonas aeruginosa strains. Peptide alone did not have any cytotoxic activity on human fibroblasts and keratinocytes. Also it did not show any antibacterial properties and did not cause hemolysis of red blood cells. The peptide incorporated in composite showed a rapid release in the kinetics profile. The obtained results indicate that there is the technological possibility to incorporate peptides in chitosan/ZnO‐doped bioglass scaffolds. Such biomaterials have potential application in bone tissue engineering.

中文翻译：

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多孔壳聚糖/ ZnO掺杂生物玻璃复合物作为生物活性肽的载体

在这项研究中，我们旨在评估是否可以将壳聚糖/ ZnO掺杂的生物玻璃复合材料用作掺入生物活性肽的合适支架。制备了生物玻璃：聚合物的比例为1：1的多孔复合材料，并将其用作传递肽的基质。选择具有PEPTIDES序列的肽（Pro-Glu-Pro-Thr-Ile-Asp-Glu-Ser）作为模型肽。评估了壳聚糖/ ZnO掺杂生物玻璃的微观结构和孔径。复合材料的开孔率和孔径适合于使细胞迁移并确保在植入物内轻松输送营养。此外，复合材料对金黄色葡萄球菌和铜绿假单胞菌具有生物活性和杀菌活性。株。单独的肽对人成纤维细胞和角质形成细胞没有任何细胞毒活性。它也没有显示任何抗菌特性，也没有引起红细胞溶血。掺入复合物中的肽在动力学曲线中显示出快速释放。获得的结果表明，在壳聚糖/ ZnO掺杂的生物玻璃支架中掺入肽存在技术可能性。这种生物材料在骨组织工程中具有潜在的应用。