Ginkgo biloba L. leaf (GBL) is one of the most commonly used medicinal plants in the world. Phenolic acids with biological activities have a relatively high content in G. biloba leaf extracts (GBE); therefore they are of great significance for the quality control of GBL, GBE and its preparations. However, there have been few studies focused on their analysis. In this work, 12 phenolic acids, including 11 phenolic acid glycosides, were identified by liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (LC–Q‐TOF/MS). Then, a method combining enzymolysis with HPLC was established for quantification of phenolic acid glycosides. It was found that the aglycones of phenolic acid glycosides mainly comprised five phenolic acids: 2,4,6‐trihydroxybenzoic acid, protocatechuic acid, p‐hydroxybenzoic acid, vanillic acid and p‐coumaric acid. The quantitative method was validated, and the correlation coefficient (0.9993–0.9999), recovery (≥88.4%), repeatability (≤0.8%), and inter‐day precision (≤5.5%) were satisfactory. Finally, the contents of glycosides of five phenolic acids in GBL, GBE and GBE injection from different sources were determined by the developed method. The method was accurate, repeatable and practicable, which could be helpful for the quantification of phenolic acid glycosides in other products containing GBL or GBE.

中文翻译：

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银杏叶，银杏叶提取物及其注射液中酚酸苷的定性和定量分析

银杏叶（GBL）是世界上最常用的药用植物之一。具有生物活性的酚酸在银杏叶提取物中含量较高。因此，它们对于GBL，GBE及其制剂的质量控制具有重要意义。但是，很少有研究专注于其分析。在这项工作中，通过液相色谱结合四极杆飞行时间质谱（LC-Q-TOF / MS）鉴定了12种酚酸，包括11种酚酸糖苷。然后，建立了一种将酶解与HPLC相结合的方法来定量酚酸糖苷。发现酚酸糖苷的糖苷配基主要包含5种酚酸：2,4,6-三羟基苯甲酸，原儿茶酸，对羟基苯甲酸，香草酸和对香豆酸。定量方法得到了验证，相关系数（0.9993–0.9999），回收率（≥88.4％），重复性（≤0.8％）和日间精度（≤5.5％）令人满意。最后，通过改进方法测定了不同来源的GBL，GBE和GBE注射液中5种酚酸的糖苷含量。该方法准确，可重复，实用，对定量分析其他含有GBL或GBE的产品中酚酸苷的含量可能有帮助。