Cardiovascular disease is one of the major causes of deaths worldwide. Increased arginase activity is associated with cardiovascular disease. The literature shows that plants are a good source of arginase inhibitors. Hence in the present work arginase inhibitor activity is studied from Ficus religiosa leaves. A fine powder of F. religiosa leaves was serially extracted in various solvents, viz. hexane, chloroform, ethyl acetate and methanol. Out of those four solvent extracts, the one showing highest arginase inhibitor activity was loaded onto the column for further fractionation. Among the collected fractions, the one showing the highest activity was subjected to identification of metabolites by using LC–HRMS. Total compounds including acipimox, edoxudine, levulinic acid, hydroxyhydroquinone, ramiprilglucuronide, berberine, antimycin A, swietenine and some short peptides were identified from the fraction showing the highest arginase inhibitory activity. Identification of these metabolites from F. religiosa and their biological importance may help to promote its use as medicinal plant. Further purification and characterization of therapeutically novel molecules will be the subject of future work.

中文翻译：

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LC-HRMS精氨酸酶抑制剂活性指导的榕树叶片的代谢产物谱分析。

心血管疾病是全世界死亡的主要原因之一。精氨酸酶活性增加与心血管疾病有关。文献表明植物是精氨酸酶抑制剂的良好来源。因此，在本工作中，从菩提榕叶研究了精氨酸酶抑制剂的活性。菩提树细粉在各种溶剂中连续提取叶子。己烷，氯仿，乙酸乙酯和甲醇。在这四种溶剂提取物中，将显示出最高精氨酸酶抑制剂活性的一种提取物上样到色谱柱上进行进一步分离。在收集的馏分中，显示最高活性的馏分通过LC-HRMS进行代谢物鉴定。从显示出最高精氨酸酶抑制活性的馏分中鉴定出总化合物，包括阿西莫克斯，依多西定，乙酰丙酸，羟基氢醌，雷米普葡糖醛酸，小ber碱，抗霉素A，瑞汀和一些短肽。从菩提树中鉴定这些代谢产物并且它们的生物学重要性可能有助于促进其作为药用植物的用途。治疗性新分子的进一步纯化和表征将是未来工作的主题。