Barbour‐Stoenner‐Kelly II (BSK‐II) and BSK‐H media were used for cultivation and isolation of fastidious Borrelia species ‐ the causative agents of Lyme borreliosis. Culture media have a limited shelf life and require adequate storage. Our goal was to assess how the growth of Borrelia would be affected by prolonged storage of media and inadequate storage conditions (BSK‐H stored at +4 °C for 2.5 years and BSK‐II stored at −20 °C for 11 years). Growth of different Borrelia afzelii, Borrelia garinii, Borrelia lusitaniae and Borrelia valaisiana strains was assessed during 2 weeks of incubation at 33 °C. Monitored parameters included cell count per mL, morphology and motility. The results of this study have shown weaker growth of borrelia strains in BSK‐H at +4 °C (median final cell number of 1.5 × 10⁶/mL) than in BSK‐II at −20 °C (median final cell number of 7.75 × 10⁶/mL) and in fresh BSK‐H media (median final cell number of 8.95 × 10⁶/mL). Duration of storage of media had no impact on Borrelia morphology and motility. Our results indicate that temperature of −20 °C is optimal for long‐term storage of medium, BSK‐II stored for 11 years provided effective support to growth of Borrelia and may be employed for cultivation.

中文翻译：

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长时间存放的BSK-H和BSK-II培养基中的疏螺旋体的生长和形态的比较。

Barbour-Stoenner-Kelly II（BSK-II）和BSK-H培养基用于培养和分离难养的疏螺旋体-莱姆疏螺旋体病的病原体。培养基的保存期限有限，需要足够的存储空间。我们的目标是评估培养基的长时间存储和不适当的存储条件（BSK-H在+4°C下存储2.5年，BSK-II在-20°C下存储11年）将如何影响Borrelia的生长。不同的成长疏螺旋体afzelii，疏螺旋体garinii，伯氏疏葡萄牙念珠菌和伯氏疏valaisiana在33°C孵育2周的过程中评估了这些菌株。监测的参数包括每毫升细胞数，形态和运动性。这项研究的结果表明，在+4°C（中位数最终细胞数为1.5×10 ⁶ / mL）的BSK‐H中，疏螺旋体菌株的生长较在−20°C（BSC–H中最终细胞数的中位数）弱7.75×10 ⁶ / mL）和新鲜的BSK-H培养基（最终细胞中位数为8.95×10 ⁶ / mL）。培养基的保存时间对疏螺旋体的形态和运动性没有影响。我们的结果表明，-20°C的温度对于培养基的长期储存是最佳的，BSK-II储存11年可为疏螺旋藻的生长提供有效支持，并可用于种植。