Proof‐of‐Principle Experiment of the Pseudorandom Multiplexing of White‐Pump Light for Spectral Photothermal Microscopy,Annalen Der Physik

当前位置： X-MOL 学术 › Ann. Phys. (Berlin) › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Proof‐of‐Principle Experiment of the Pseudorandom Multiplexing of White‐Pump Light for Spectral Photothermal Microscopy
Annalen Der Physik ( IF 2.2 ) Pub Date : 2020-08-13 , DOI: 10.1002/andp.202000241
Keisuke Seto ₁ , Hiromu Yamada ₁ , Takayoshi Kobayashi ₂ , Eiji Tokunaga ₁

Affiliation

Absorption spectroscopy, coupled with photothermal super‐resolution microscopy, is enabled by the frequency division multiplexing of the pump‐light (Pu) wavelength whose spectral component is discriminated by the modulation frequency. This demonstrates a great advantage in availing multicolor image information. However, the response gain of the thermal lens signal to the spectral components is not uniform because of the limited relaxation rate of the thermal lens, i.e., the gains of the components assigned to the high‐frequency modulations are diminished, thus resulting in a distorted absorption spectrum. In this study, white‐pump light (Pu) is multiplexed by pseudonoise sequences (PNSs) to avoid the distortion. The frequency bandwidth of the PNSs is equalized, and the gains of the thermal lens generation to the respective wavelength components are balanced. The obtained results indicate that the modulation‐waveform deformation due to the slow responses of the sample can also distort spectra. To mitigate the problem, the countermeasures required to compensate for this distortion are also discussed.

中文翻译：

光谱光热显微镜中白泵浦光的伪随机多路复用原理验证实验

通过泵浦光（Pu）波长的频分复用实现吸收光谱与光热超分辨率显微镜的结合，其光谱成分由调制频率来区分。这证明了在利用多色图像信息方面的巨大优势。但是，由于热透镜的弛豫率有限，热透镜信号对频谱分量的响应增益不均匀，即分配给高频调制的分量的增益减小，从而导致失真吸收光谱。在这项研究中，白泵浦光（Pu）与伪噪声序列（PNSs）复用以避免失真。PNS的频率带宽相等，并且热透镜产生对各个波长分量的增益是平衡的。获得的结果表明，由于样品响应慢而导致的调制波形变形也会使光谱失真。为了减轻该问题，还讨论了补偿这种失真所需的对策。

更新日期：2020-08-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11