Poultry litter is used as soil amendment or organic fertilizer. While poultry litter is enriched with organic matter suitable for land, the presence of pathogens such as Salmonella in poultry litter is a concern. To investigate the effect of gaseous ozone on pathogen reductions in poultry litter, this study conducted a series of experiments that involved understanding of Salmonella Typhimurium and Escherichia coli O157:H7 inactivation at various doses of Ozone (O₃) in wet and dry poultry litter conditions. Previously, ozone treatment has been shown to disinfect the surface of foods and plant materials including fruits, juices, and wastewater, however, additional research are needed to better understand the impacts of ozone on treatment of soil amendments. Sanitizing methods capable of eliminating pathogens of soil amendments are crucial to mitigate disease outbreaks related with litter/manure-based fertilizers. In this study, a bench scale continuous ozone treatment system was designed to produce O₃ gas, with a range O₃ concentrations (7.15–132.46 mg·L⁻¹), monitor ozone concentrations continuously, and control the ozone exposure time (15 to 90 mins) to understand the effectiveness of O₃ in eliminating S. Typhimurium and E. coli O157:H7 in poultry litter. Results showed that 7.15 mg·L^-1 did not reduce the counts of S. Typhimurium until exposure to O₃ for 90 min. The O₃ concentrations of 43.26 ~ 132.46 mg·L^-1 exposure reduced the bacterial counts. Furthermore, the moisture content of poultry litter was found to be an influencing factor for pathogen reduction. The pathogen reduction rates were reduced when the moisture content was increased. At higher moisture content, high concentrations of O₃ (132.46 mg·L^-1) were needed for pathogen reductions. The moisture content of 30% or lower was found to be more effective for controlling pathogen levels in poultry litter. Our study demonstrates that gaseous O₃ treatment could be used as an additional decontamination technique to ensure the certain degree of microbiological safety of poultry litter based soil amendment.

家禽垫料用作土壤改良剂或有机肥料。尽管家禽垃圾中富含适用于土地的有机物质，但家禽垃圾中是否存在诸如沙门氏菌等病原体也是一个问题。为了研究气态臭氧对家禽垫料中病原体减少的影响，本研究进行了一系列实验，涉及了解不同剂量的臭氧（O ₃引起鼠伤寒沙门氏菌和大肠杆菌O157：H7的灭活））在干和湿的家禽垫料条件下。以前，已经证明臭氧处理可以对食品和植物材料的表面（包括水果，果汁和废水）进行消毒，但是，还需要进行更多的研究以更好地了解臭氧对土壤改良剂处理的影响。能够消除土壤改良剂病原体的消毒方法对于减轻与垃圾/肥料基肥料相关的疾病暴发至关重要。在这项研究中，工作台规模连续臭氧处理系统被设计成产生Õ ₃气体，用范围ø ₃浓度（7.15-132.46毫克·L ^-1），监视器臭氧浓度连续地，并控制臭氧暴露时间（15到90分钟）以了解O ₃的有效性在消除S.鼠伤寒和大肠杆菌O157：H7的家禽废弃物。结果表明，7.15毫克·L ^-1没有降低的计数S.鼠伤寒沙门氏菌，直到曝光至O ₃ 90分钟。暴露于43.26〜132.46 mg·L ^-1的O ₃浓度降低了细菌数量。此外，发现家禽垫料的水分含量是减少病原体的影响因素。当水分含量增加时，病原体减少率降低。在较高的水分含量下，高浓度的O ₃（132.46 mg·L ^-1）是减少病原体所必需的。发现水分含量为30％或更低对于控制家禽垫料中的病原体水平更有效。我们的研究表明，气态O ₃处理可以用作附加的去污技术，以确保家禽垫料土壤改良剂在一定程度上具有微生物安全性。