Phomopsisin A–C (1–3), three new leucine-derived cytochalasan alkaloids bearing methylation deficient skeleton were isolated from Phomopsis sp. sh917, an endophytic fungus colonized in the stems of Isodon eriocalyx var. laxiflora. Their structures and absolute configurations were determined through synergetic use of extensive spectroscopic analysis, quantum chemical calculations, and X-ray crystallographic analysis. Noteworthily, 1 represents the first example of cytochalasan possessing an unusual 2H-isoxazole moiety. Additionally, their cytotoxic and anti-inflammatory activities were initially evaluated, and 3 showed a moderate inhibitory effect against the NO production.

Phomopsisin A-C（1 - 3），三个新的亮氨酸衍生cytochalasan生物碱轴承甲基化缺陷型骨架从分离拟茎点霉属。sh917，的内生真菌在定植茎的香茶eriocalyx变种 Laxiflora。通过协同使用广泛的光谱分析，量子化学计算和X射线晶体学分析，确定了它们的结构和绝对构型。值得注意的是，1代表具有不寻常的2H-异恶唑部分的细胞松弛素的第一个例子。此外，还初步评估了它们的细胞毒性和抗炎活性，并且3 对NO产生适度的抑制作用。