Abstract Chitosan (Cs) mats obtained by electrospinning are potentially ideal scaffolds for tissue engineering. This technique allows obtaining nanometric fibrous structures with preferred orientation, which in turn enable cells to align themselves and produce extracellular matrix along desired orientations. In this study, we fabricated aligned Cs electrospun nanofiber mats and investigated the role of the amino acid l -Arginine (L-Arg) as stabilizing agent. Morphological, chemical, mechanical and biological characterizations were performed on untreated and L-Arg treated nanofibrous mats showing the role of L-Arg as biomimetic stabilizer. L-Arg acts as chemical stabilizer of nanofibrous mats, providing improved wettability behavior, mechanical properties and stability even after 60 days in aqueous medium in comparison to untreated mats. Moreover, preliminary biological tests demonstrated favorable cell-material interactions implying physiological responses in terms of viability and proliferation. The proposed L-Arg-treated Cs mats can be considered as potential scaffolds for highly oriented tissue patterning.

中文翻译：

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L-精氨酸处理对电纺定向壳聚糖纳米纤维垫体外稳定性的影响

摘要 通过静电纺丝获得的壳聚糖 (Cs) 垫可能是组织工程的理想支架。该技术允许获得具有首选方向的纳米纤维结构，这反过来又使细胞能够排列自己并沿着所需的方向产生细胞外基质。在这项研究中，我们制造了对齐的 Cs 电纺纳米纤维垫，并研究了氨基酸 l-精氨酸 (L-Arg) 作为稳定剂的作用。对未处理和 L-Arg 处理的纳米纤维垫进行形态学、化学、机械和生物学表征，显示 L-Arg 作为仿生稳定剂的作用。L-Arg 作为纳米纤维垫的化学稳定剂，与未处理的垫相比，即使在水性介质中 60 天后也能提供更好的润湿性、机械性能和稳定性。而且，初步的生物学测试表明有利的细胞-材料相互作用意味着在活力和增殖方面的生理反应。拟议的 L-Arg 处理的 Cs 垫可以被认为是高度定向组织图案的潜在支架。