We describe two new species of deer truffle, Elaphomyces marmoratus and E. fuscus spp. nov., collected from a secondary forest dominated by Quercus serrata, in Tokyo, Japan. Both species have morphological similarities to E. muricatus and E. granulatus; however, the former has a gleba without a purplish color and the latter has reticulated ascospores more closely resembling E. asperulus. Molecular phylogenetic analyses also support the assignment of these specimens as new species. ITS rDNA homologies with known species were low (<91% and <97%). In addition, phylogenetic trees using neighbor-joining and maximum likelihood methods showed that the sequences of the two new species each formed a monophyletic group within section Elaphomyces with bootstrap support of 99%. Analyses of ectomycorrhizal roots collected concurrently with the ascomata revealed that E. marmoratus is associated with at least Pinus densiflora, while E. fuscus is associated with at least Q. serrata.

我们描述鹿松露的两个新品种，Elaphomyces菖和ē。桑属 十一月，采自日本东京以栎栎为主的次生林。两种物种都与E形态相似。刺果和Ë。颗粒体; 但是，前者有没有紫色的颜色gleba，后者已网状子囊孢子更紧密地类似ê。曲霉菌。分子系统发育分析也支持将这些标本指定为新物种。已知物种的ITS rDNA同源性较低（<91％和<97％）。此外，使用近邻结合和最大似然方法的系统发育树显示，这两个新物种的序列各自在衣藻科中形成了单系群，自举支持率为99％。与ascomata同时收集外生菌根根的分析显示，Ë。鳢与至少相关的赤松，而ē。us至少与Q有关。锯齿状。