Dental stem cells have great potential in clinical practice as an adult mesenchymal stem cell, such as dental follicle and the apical papilla, have strong proliferation and differentiation characteristics. The developmental relevance and discrimination of them in the niche is not clear, which limits their application scenarios. The aim of this study was to investigate the intrinsical differences in cellular contents of DFSCs and SCAP by Tandem mass tag (TMT) labeling quantitative proteomics. Cell lysates were labeled and tracked by the combined use of TMT and LC-MS/MS. A total of 1622 proteins were detected, of which 421 were different and 12 were significantly up-regulated and 4 were significantly down-regulated. The results of proteomics support the application of stem cells in the treatment of neurodegenerative diseases such as Huntington's disease, Alzheimer's disease, Parkinson's disease and so on. The difference is related to cell proliferation and protection of neurons from inflammation and autophagy damage. Highly expressed proteins predict the special ability of DFSCs to stably proliferate and differentiate through CD13, MARCKS, and PAST1. The strong immune stability of SCAP is supported by NPC1.This study expands our understanding on the molecular mechanisms of tooth development and regeneration, and provide basic support for dental stem cells in clinical applications such as neurological and immune diseases.

牙科干细胞在临床上具有巨大的潜力，因为成年的间充质干细胞，例如牙囊和根尖乳头，具有很强的增殖和分化特性。在利基市场中它们的发展相关性和区别尚不清楚，这限制了它们的应用场景。本研究的目的是通过串联质量标签（TMT）标记定量蛋白质组学研究DFSCs和SCAP细胞含量的内在差异。通过同时使用TMT和LC-MS / MS标记和追踪细胞裂解液。总共检测到1622种蛋白质，其中421种不同，其中12种显着上调，而4种显着下调。蛋白质组学的研究结果支持干细胞在诸如亨廷顿病等神经退行性疾病的治疗中的应用 氏病，阿尔茨海默氏病，帕金森氏病等。差异与细胞增殖以及保护神经元免受炎症和自噬损害有关。高表达蛋白预测DFSC通过CD13，MARCKS和PAST1稳定增殖和分化的特殊能力。NCAP1支持SCAP强大的免疫稳定性。这项研究拓宽了我们对牙齿发育和再生的分子机制的了解，并为牙齿干细胞在神经和免疫疾病等临床应用中提供了基础支持。高表达蛋白预测DFSC通过CD13，MARCKS和PAST1稳定增殖和分化的特殊能力。NCAP1支持SCAP的强大免疫稳定性。这项研究拓宽了我们对牙齿发育和再生的分子机制的了解，并为牙齿干细胞在神经和免疫疾病等临床应用中提供了基础支持。高表达蛋白预测DFSC通过CD13，MARCKS和PAST1稳定增殖和分化的特殊能力。NCAP1支持SCAP强大的免疫稳定性。这项研究拓宽了我们对牙齿发育和再生的分子机制的了解，并为牙齿干细胞在神经和免疫疾病等临床应用中提供了基础支持。