The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968. This review examines the major developments and trends that have occurred in this technique over the past five decades. The basic principles and history of this area are first discussed. This is followed by an overview of the various supports, immobilization strategies, and types of binding agents that have been used in this field. The general types of applications and fields of use that have appeared for affinity chromatography are also considered. A survey of the literature is used to identify major trends in these topics and important areas of use for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.

亲和色谱领域采用生物相关试剂作为固定相，自 1968 年该方法的现代时代开始以来，该领域取得了显着的发展。本综述探讨了该技术在过去的主要发展和趋势五个十年。首先讨论该领域的基本原理和历史。接下来概述了该领域已使用的各种支持物、固定策略和结合剂类型。还考虑了亲和色谱的一般应用类型和使用领域。通过文献调查来确定这些主题的主要趋势以及亲和色谱在化学品和生物化学品的分离、分析或表征中的重要使用领域。