Abstract In this work, we have developed a sensitive, flexible, cost-effective Ag/Nanocellulose fibers SERS substrate (Ag@NCF substrate) which is suitable for in-situ food safety detection. NCFs build nanoporous networks as flexible templates. Ag nanoparticles time-efficiently, in-situ grow on the cross fibers, forming sensitive Ag clusters, providing a high density of ‘hot-spot’, further resulting in a significant enhancement of electromagnetic fields. The lowest distinguishable level of Rhodamine 6G is 10−9 M and simplified models correspondingly reveal a high sensitivity of Ag@NCF substrate. In additions, the Ag@NCF film facilitates a simple, direct, in-situ ‘paste-test’ method for hazardous residue detection on actual food samples. The sensitive Ag@NCF substrates achieve ppb-level detection of pesticide and veterinary drug by a self-developed portable Raman spectrometer. The estimated LOD is low to 0.05 ppm for thiram, 0.09 ppm for thiabendazole, 0.0014 ppm for malachite green and 0.069 ppm for enrofloxacin, respectively. And hazardous residues could be directly detected on pear and fish by the Ag@NCF substrates. These results reveal that Ag@NCF substrate cooperated with portable Raman system is suitable for sensitive, in-situ, on-site pesticides and veterinary drugs detection on fruit or seafood and remains great potentials to expand to other on-site, in-situ applications.

中文翻译：

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柔性银/纳米纤维素纤维 SERS 基材及其在食品原位危险残留检测中的应用

摘要 在这项工作中，我们开发了一种灵敏、灵活、具有成本效益的 Ag/纳米纤维素纤维 SERS 基板（Ag@NCF 基板），适用于原位食品安全检测。NCF 构建纳米多孔网络作为灵活的模板。银纳米粒子在交叉纤维上原位生长，形成敏感的银簇，提供高密度的“热点”，进一步显着增强了电磁场。罗丹明 6G 的最低可区分水平为 10-9 M，简化模型相应地揭示了 Ag@NCF 基板的高灵敏度。此外，Ag@NCF 薄膜促进了一种简单、直接、原位“糊状测试”方法，用于实际食品样品的危险残留检测。灵敏的Ag@NCF底物通过自主研发的便携式拉曼光谱仪实现了农药和兽药的ppb级检测。估计的 LOD 分别为福美双 0.05 ppm、噻菌灵 0.09 ppm、孔雀石绿 0.0014 ppm 和恩诺沙星 0.069 ppm。Ag@NCF 底物可以直接检测梨和鱼上的有害残留物。这些结果表明，Ag@NCF 底物与便携式拉曼系统配合使用适用于水果或海产品的敏感、原位、现场农药和兽药检测，并且仍有很大的潜力扩展到其他现场、原位应用. Ag@NCF 底物可以直接检测梨和鱼上的有害残留物。这些结果表明，Ag@NCF 底物与便携式拉曼系统配合使用适用于水果或海产品的敏感、原位、现场农药和兽药检测，并且仍有很大的潜力扩展到其他现场、原位应用. Ag@NCF 底物可以直接检测梨和鱼上的有害残留物。这些结果表明，Ag@NCF 底物与便携式拉曼系统配合使用适用于水果或海产品的敏感、原位、现场农药和兽药检测，并且仍有很大的潜力扩展到其他现场、原位应用.